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失调的中性粒细胞在败血症中形成细胞外陷

Maria Mulet¹, Ruben Osuna-Gómez¹, Carlos Zamora¹

¹Department of Inflammatory Diseases, Institut Recerca Hospital de la Santa Creu i Sant Pau, Barcelona, Spain.

|June 29, 2023

概括

败血症会损害中性粒细胞的功能,包括减少中性粒细胞细胞外陷 (NET) 的形成. 中性细胞CD11b表达和NETosis可以帮助区分败血症和非败血症患者,这表明败血症期间病原体消除受损.

科学领域:

免疫学免疫学免疫学
关键护理医学关键护理医学
病原体与宿主之间的相互作用

背景情况:

中性粒细胞迁移和抗菌功能在败血症中经常受到损害,导致免疫失调.
中性粒细胞外细胞陷 (NETs) 在败血症发病过程中的特定作用需要进一步澄清.

研究的目的:

为了分析中性粒细胞表型和功能在败血症诊断后的连续变化.
调查中性粒细胞特征,NET形成和败血症结果之间的关系.

主要方法:

预期招募败血症患者 (n=49),非败血症患者 (n=18) 和健康志愿者 (n=20).
血液样本采集在基线,24,48,和72小时后的败血症诊断.
评估中性粒细胞的表型 (CD66b,CD11b,CD177表达) 和通过流细胞计进行脱粒化.
使用光和分析中性粒细胞-血小板相互作用来量化NET形成.

主要成果:

败血性中性粒细胞显示CD66b,CD11b和CD177表达增加,但与对照组相比,NET形成减少.
对中性粒细胞的CD177表达与减少NETosis和较差的败血症结果相关.
实验室研究表明,败血症起源 (病原体类型,受影响的器官) 损害了中性粒细胞的功能.
一个决策树模型确定了CD11b表达和NETosis作为败血症和非败血症患者之间的关键歧视因素.

结论:

败血症显著改变中性粒细胞的表型和功能,可能会损害宿主消除病原体的能力.
CD11b表达和NETosis水平作为毒症诊断的有价值的生物标志物.
了解这些中性粒细胞的动态对于治疗败血症和改善患者的治疗结果至关重要.

关键词:

CD177 是一个CD177 是一个CD177 网症 (NETosis) 是一种疾病.作为一个中性恋者.这是一种血症.

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