来自智利的特有虫中的四体:南美洲的分子证据
J P Correa1, F Farías2, E San Juan2
1Facultad de Ciencias de la Naturaleza, Universidad San Sebastián, Concepción, Chile.
Journal of helminthology
|June 29, 2023
概括
这项研究确定了智利有争议的带状虫Mesocestoides的新中间宿主,为新热带地区的这种寄生虫提供了第一个分子数据,并澄清了它的进化关系.
科学领域:
- 寄生虫学的寄生虫学
- 分子生物学分子生物学
- 类学 类学 类学 类学
背景情况:
- 带状属Mesocestoides对系统学和生命周期的理解很差.
- 脊椎动物,特别是食肉哺乳动物,是最终宿主,节肢动物和其他脊椎动物是中间宿主.
- 最近的证据表明,简化了两种宿主生命周期,新热带地区的分子数据缺乏.
研究的目的:
- 为了确定一个额外的中间宿主,在新热带地区的Mesocestoides.
- 单独的幼虫形式的分子特征的Mesocestoides.
- 为了解属的进化关系和生命周期做出贡献.
主要方法:
- 在智利北部收集并剖析了18只编织树木 (Liolaemus platei).
- 带状幼虫的形态鉴定为Mesocestoides tetrathyridia. 这种类型的幼虫是
- 使用常规聚合酶链反应 (cPCR) 的分子分析来放大18S rRNA和12S rRNA的位置.
主要成果:
- 一只被发现感染了三种类型的Mesocestoides幼虫.
- 遗传学分析证实幼虫属于Mesocestoides,并且是同物种.
- 这些序列形成了一个单体类,是Mesocestoides类C的姐妹,代表了新热带地区这个属的第一个分子数据.
结论:
- 这项研究提供了新热带地区中类动物的第一个分子特征,确定了Liolaemus platei作为一种新的中间宿主.
- 这些发现支持不同幼虫形态类型的共同特异性.
- 需要对最终宿主和整合性分类学进行进一步的研究,以充分阐明该地区的Mesocestoides生命周期和进化历史.
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