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相关概念视频

Chromatin Packaging01:32

Chromatin Packaging

16.8K
Each human somatic cell contains 6 billion base pairs of DNA. Each base pair is 0.34 nm long, meaning each diploid cell contains a staggering 2 meters of DNA. This long DNA strand is packed inside a nucleus measuring only 10-20 microns in diameter with the help of specialized DNA-binding proteins called histones. Together they form a compact DNA-protein complex called chromatin. The chromatin is further compacted into higher-order structures. The highest level of compaction is achieved during...
16.8K
Chromatin Immunoprecipitation- ChIP02:36

Chromatin Immunoprecipitation- ChIP

11.2K
Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...
11.2K
Duplication of Chromatin Structure02:05

Duplication of Chromatin Structure

5.6K
The process of chromosome duplication during cell division requires genome-wide disruption and re-assembly of chromatin. The chromatin structure must be accurately inherited, reassembled, and maintained in the daughter cells to ensure lineage propagation.
The basic unit of the chromatin is the nucleosome, consisting of DNA wrapped around octameric histone proteins and short stretches of linker DNA separating individual nucleosomes. The histone proteins within the nucleosome have their...
5.6K
Spreading of Chromatin Modifications02:25

Spreading of Chromatin Modifications

8.3K
The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
8.3K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

7.1K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
7.1K
Chromatin Position Affects Gene Expression02:35

Chromatin Position Affects Gene Expression

23.4K
Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences  access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area. 
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
23.4K

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相关实验视频

Updated: Jul 25, 2025

Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C
09:32

Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C

Published on: October 14, 2022

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iEnhance:一个多尺度空间投影编码网络,用于增强染色体相互作用数据分辨率.

Kai Li1, Ping Zhang1, Zilin Wang1

  • 1Hubei Key Laboratory of Agricultural Bioinformatics, College of Informatics, Huazhong Agricultural University, Wuhan 430070, China.

Briefings in bioinformatics
|June 29, 2023
PubMed
概括
此摘要是机器生成的。

iEnhance增强了低分辨率的染色质相互作用数据,以揭示精确的3D基因组架构. 这种人工智能工具可以提高结构元素的准确性,从而更好地了解基因组组织.

关键词:
染色素相互作用数据染色体循环的染色体循环是什么深度学习是一种深度学习.模型评价模型评价拓学关联领域 拓学关联领域

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Capturing Chromosome Conformation Across Length Scales
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Capturing Chromosome Conformation Across Length Scales

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High-Resolution Mapping of Protein-DNA Interactions in Mouse Stem Cell-Derived Neurons using Chromatin Immunoprecipitation-Exonuclease ChIP-Exo
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High-Resolution Mapping of Protein-DNA Interactions in Mouse Stem Cell-Derived Neurons using Chromatin Immunoprecipitation-Exonuclease ChIP-Exo

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相关实验视频

Last Updated: Jul 25, 2025

Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C
09:32

Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C

Published on: October 14, 2022

3.5K
Capturing Chromosome Conformation Across Length Scales
10:15

Capturing Chromosome Conformation Across Length Scales

Published on: January 20, 2023

3.5K
High-Resolution Mapping of Protein-DNA Interactions in Mouse Stem Cell-Derived Neurons using Chromatin Immunoprecipitation-Exonuclease ChIP-Exo
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High-Resolution Mapping of Protein-DNA Interactions in Mouse Stem Cell-Derived Neurons using Chromatin Immunoprecipitation-Exonuclease ChIP-Exo

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科学领域:

  • 基因组学就是基因组学.
  • 计算生物学 计算生物学
  • 生物信息学是一种生物信息学.

背景情况:

  • 高通量染色体相互作用数据对于理解3D基因组架构至关重要.
  • 数据稀疏性和高信号噪声比限制了从这些方法中获得的结构元素的精度.
  • 现有的工具很难准确地恢复短程和长程交互模式.

研究的目的:

  • 开发一种新的计算工具iEnhance,用于提高染色体相互作用数据的分辨率.
  • 通过克服数据限制,提高3D基因组结构元素的精度.
  • 创建适用于各种染色体相互作用数据集和细胞类型的多功能工具.

主要方法:

  • iEnhance采用一个多尺度的空间投影和编码网络.
  • 它使用矩阵投影从输入数据中提取多个规模的全球和本地特征.
  • 功能通过注意力机制在层次上融合,其次是密集通道编码和剩余通道解码.

主要成果:

  • iEnhance显著优于现有的最先进的Hi-C分辨率增强工具.
  • 该方法精确地恢复了短距离结构元素和长距离相互作用模式.
  • iEnhance在各种数据集中表现出强大的性能,包括单细胞Hi-C和Micro-C,并可转移到新的细胞系.

结论:

  • iEnhance为高分辨率染色体相互作用数据增强提供了强大而准确的方法.
  • 该工具克服了稀疏和杂数据的局限性,允许更精确的3D基因组架构分析.
  • iEnhance为各种基因组研究提供了广泛的适用性和可转移性.