米的运输体OsHAK18调解了K的载荷和再分配
Like Shen1, Wenxia Fan1, Na Li1
1State Key Laboratory of Crop Genetics and Germplasm Enhancement and Utilization, College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, China.
The Plant journal : for cell and molecular biology
|June 29, 2023
概括
高亲和度运输体 (HAK/KUP/KT) 对植物平衡至关重要. 这项研究揭示了OsHAK18
科学领域:
- 植物生理学 植物生理学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 高亲和力运输体 (HAK/KUP/KT) 对植物的 (K+) 稳态至关重要,影响生长和应激反应.
- 虽然它们在根K+吸收和运输到芽中的作用已经确立,但它们在花K+转移中的功能仍然基本上是未知的.
研究的目的:
- 研究体局部化大米运输体OsHAK18在K+运输中的作用,特别是体转移.
- 为了确定OsHAK18中断对大米植物对低 (LK) 压力的反应的影响.
主要方法:
- 通过酵母,大肠杆菌和阿拉比多普西斯中的表达来描述OsHAK18的功能特征.
- 对OsHAK18在血中的定位的分析.
- 在LK压力下对大米oshak18突变系的表型分析,包括K+含量测量和分裂根测试.
主要成果:
- OsHAK18调解K+吸收,并且局部存在于血膜中.
- 破坏OsHAK18使得对LK压力缺乏敏感性,突变者表现出减少色和化.
- oshak18突变体表现出K+分布的改变,在LK压力下,在芽中积累更多的K+,在根中积累更少的K+,具有降低的体K+度和抑制的根到芽到根K+转移.
结论:
- OsHAK18在米中的K+载体和再分配中起着重要作用.
- 破坏OsHAK18在低K+条件下增强射击K+保留,改善对K+缺乏的耐受性.
- 这项研究扩大了对HAK/KUP/KT载体的功能理解,并建议OsHAK18作为改善米K+缺陷耐受性的目标.
更多相关视频
12:48Measuring Cation Transport by Na,K- and H,K-ATPase in Xenopus Oocytes by Atomic Absorption Spectrophotometry: An Alternative to Radioisotope Assays
Published on: February 19, 2013
10.6K
05:47Phosphoproteomic Strategy for Profiling Osmotic Stress Signaling in Arabidopsis
Published on: June 25, 2020
5.2K
相关概念视频
01:17Reabsorption and Secretion in the Loop of Henle
1.6K
The thick ascending limb of the nephron loop has Na+–K+–2Cl− symporters in the apical membranes of its cells. These symporters simultaneously reclaim one sodium ion, one potassium ion, and two chloride ions from the tubular fluid. Sodium ions are actively transported into the interstitial fluid at the base and sides of the cell, diffusing into the vasa recta. Chloride ions move through leakage channels in the basolateral membrane into the interstitial fluid and then into the...
1.6K
02:02Phloem and Sugar Transport
37.6K
Like many living organisms, plants have tissues that specialize in specific plant functions. For example, shoots are well adapted to rapid growth, while roots are structured to acquire resources efficiently. However, sugar production is primarily restricted to the photosynthetic cells that reside in the leaves of angiosperm plants. Sugar and other resources are transported from photosynthetic tissues to other specialized tissues by a process called translocation.
37.6K
01:29Primary Active Transport
10.4K
In contrast to passive transport, active transport involves a substance being moved through membranes in a direction against its concentration or electrochemical gradient. There are two types of active transport: primary active transport and secondary active transport. Primary active transport utilizes chemical energy from ATP to drive protein pumps embedded in the cell membrane. With energy from ATP, the pumps transport ions against their electrochemical gradients—a direction they would...
10.4K
01:14Active Transport
763
Active transport is a critical biological process that allows cells to move solutes against an electrochemical gradient. This process requires direct energy input and is characterized by its selectivity, saturability, and susceptibility to competitive inhibition.
Primary active transporters, like Na+, K+ and -ATPase, directly utilize ATP to move ions across the membrane. These transporters play significant roles in various physiological processes. For instance, Na+, K+ and -ATPase maintain...
Primary active transporters, like Na+, K+ and -ATPase, directly utilize ATP to move ions across the membrane. These transporters play significant roles in various physiological processes. For instance, Na+, K+ and -ATPase maintain...
763
01:32Secondary Active Transport
7.2K
One example of how cells use the energy contained in electrochemical gradients is demonstrated by glucose transport into cells. The ion vital to this process is sodium (Na+), which is typically present in higher concentrations extracellularly than in the cytosol. Such a concentration difference is due, in part, to the action of an enzyme "pump" embedded in the cellular membrane that actively expels Na+ from a cell. Importantly, as this pump contributes to the high concentration of...
7.2K
02:03Xylem and Transpiration-driven Transport of Resources
24.2K
The xylem of vascular plants distributes water and dissolved minerals that are taken up by the roots to the rest of the plant. The cells that transport xylem sap are dead upon maturity, and the movement of xylem sap is a passive process.
24.2K