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纳米通道电注作为一种多功能平台,用于在树突细胞上高效的RNA/DNA编程

Jing Liu^1,2, Juan Jiang¹, Caiguanxi Deng¹

¹Institute of Precision Medicine, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510080, P. R. China.

Small (Weinheim an der Bergstrasse, Germany)

|June 29, 2023

概括

纳米通道电注射安全有效地将核酸输送到树突细胞 (DC) 中,用于癌症疫苗. 这种方法优化了转染效率和细胞活力,为先进的DC疫苗开发铺平了道路.

科学领域:

生物技术是生物技术.
免疫治疗是一种免疫疗法.
纳米技术纳米技术

背景情况:

树突细胞 (DC) 疫苗对于癌症免疫疗法至关重要.
将核酸有效安全地输送到DC中对于DC疫苗开发至关重要,但仍然具有挑战性.
目前的方法往往难以平衡传染效率与生物安全性和防止DC成熟.

研究的目的:

开发和优化一种新的纳米通道电注射 (NEI) 系统,以有效和安全地将核酸输入树突细胞.
评估NEI系统在传染各种核酸类型 (DNA,mRNA,circRNA) 的性能及其对DC活力和成熟的影响.
评估NEI在癌症疫苗应用中用于DC体外转化方面的潜力.

主要方法:

利用基于轨道蚀刻纳米通道膜的纳米通道电注射 (NEI) 系统.
在细胞膜上定位电场,使用纳米尺寸的通道进行低压电穿孔 (<30 V).
优化脉冲条件,以光染料,等离子体DNA,信使RNA和圆形RNA (circRNA) 感染DC2.4细胞.

主要成果:

在使用NEI的DC2.4细胞中实现了各种核酸的高转染效率 (>70%) 和生物安全性 (活力>85%) .
成功转移了初级小鼠骨髓DCs与circRNA,效率为68.3%.
证明NEI没有显著影响细胞活力或诱导初级细胞中的DC成熟.

结论:

纳米通道电注射 (NEI) 系统为体外树突细胞转化提供了安全高效的平台.
新能源研究所克服了用于DC疫苗开发的核酸输送的关键挑战.
这项技术对推进创建基于树突细胞的有效癌症疫苗具有重大前景.

关键词:

这是DNA/RNA.树突细胞是一种树突细胞.细胞内输送是细胞内输送.纳米通道电气注射的纳米通道

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