来自VSMC的维生素D受体在CKD期间调节血管化:miR-145a的潜在作用
Maite Caus1, Cristina Alonso-Montes2,3, Jose Luis Fernandez-Martin2,3
1Vascular and Renal Translational Research Group, Institute for Biomedical Research in Lleida, Spain (M.C., M.M.-A., M.B., J.M.V.).
Arteriosclerosis, thrombosis, and vascular biology
|June 29, 2023
概括
在血管光滑肌细胞 (VSMC) 中抑制局部维生素D受体 (VDR) 信号传递,可以防止慢性病 (CKD) 的血管化 (VC). 这项研究强调了miR-145a.
科学领域:
- 心血管生物学 心血管生物学
- 腎臟病學 (nephrology) 是一種醫學專業.
- 分子生物学分子生物学
背景情况:
- 血管化 (VC) 是慢性病 (CKD) 的普遍并发症,增加了患者的发病率和死亡率.
- 维生素D受体 (VDR) 在CKD相关的VC中的作用是有争议的.
- 在CKD诱导的VC期间,血管光滑肌细胞 (VSMC) 中的局部维生素D信号需要澄清.
研究的目的:
- 在CKD引起的VC的背景下,研究VSMC中本地VDR信号的作用.
- 确定VSMC中VDR抑制对CKD中VC发展的影响.
主要方法:
- 从CKD患者和对照组的上胃动脉分析.
- 在VSMC中使用CKD与条件VDR删除的小鼠模型.
- 在化条件下,用有或没有VDR的VSMC进行体外实验.
主要成果:
- 慢性病患者和小鼠表现出增加的VC和动脉VDR表达.
- 在VSMC中的有条件VDR沉默显著降低了CKD小鼠中的VC,而不改变功能或血清/酸盐.
- 在VSMC中的VDR抑制改变了骨质素,拉米尼A,硬质素和miR-145a的表达.
结论:
- 在VSMC中的本地VDR信号在CKD诱导的VC中发挥着关键作用.
- 在VSMC中抑制VDR可以防止CKD中的VC.
- miR-145a可能参与CKD中VDR介导的VC调节.
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