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[BBM基因对的生长和Panax quinquefolius中色素含量的影响]

Teng-Teng Wang¹, Jin Hu², Hong-Hong Jiao³

¹School of Traditional Chinese Medicine,Guangdong Pharmaceutical University Guangzhou 510006, China.

Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica

|June 29, 2023

概括

将婴儿潮 (BBM) 基因转移到Panax quinquefolius中显著提高了的生长速度,并增加了关键的金氏化物含量. 这项研究为改善人参提供了基础.

科学领域:

植物分子生物学植物分子生物学
生物技术是生物技术.
农业科学农业科学

背景情况:

婴儿潮 (BBM) 基因调节胚胎发育和细胞增殖等关键过程.
松 (Panax quinquefolius) 具有低效和不稳定的基因转换系统,阻碍了研究和开发.
改善人参的遗传系统对于有效的繁殖和代谢物生产至关重要.

研究的目的:

为了研究将Zea mays BBM基因转移到Panax quinquefolius callus的效果.
评估BBM基因对的生长速度和人参化物积累的影响.
为在P. quinquefolius.中建立一个高效的基因转换系统奠定基础.

主要方法:

基因枪将BBM基因输送到P. quinquefolius.
使用葡萄酸耐药性的转基因卡利的选择.
通过PCR对转基因事件的分子鉴定.
野生类型和转基因种类之间的生长率的比较分析.
使用超高性能液体染色学三重四极质谱法 (UPLC-MS/MS) 量化人参化物.

主要成果:

成功生成了四个转基因的P. quinquefolius线.
与野生类型的虫相比,转基因虫的生长速度显著更高.
在转基因细胞中,特定的金氏化物 (Rb_1,Rg_1,Ro,Re) 的积累显著增加.

结论:

BBM基因在促进P. quinquefolius的生长速度方面发挥着重要作用.
过度表达BBM基因增加了关键的金氏酸的含量.
这项研究为开发Panax物种稳定高效的基因转换协议提供了科学基础.

关键词:

这就是BBM基因.这种植物叫Panax quinquefolius.卡卢斯 (callus) 是一个.人参酸性enoside 是一种增长率增长率的增长速度是什么

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