定位激素受体表达到细胞隔间在异常性小结肠下狭窄症
Edward Ryan R Talatala1, Evan Clark1, Wenda Ye1
1Department of Otolaryngology-Head & Neck Surgery, Vanderbilt University Medical Center, Nashville, Tennessee, USA.
The Laryngoscope
|June 29, 2023
概括
异常性小囊狭窄症 (iSGS) 涉及气道纤维化,可能与女性激素有关. 这项研究在特定的呼吸道细胞中发现了雌激素和孕受体,为iSGS发展提供了洞察力.
科学领域:
- 细胞和分子生物学 细胞和分子生物学
- 耳鼻喉科 耳鼻喉科 耳鼻喉科
- 呼吸道疾病的发病因子
背景情况:
- 异常性小结节狭窄症 (iSGS) 是一种罕见的疾病,其特征是渐进的气道纤维化.
- 这种情况主要影响女性,这表明女性荷尔蒙在它的发展中发挥了作用.
- 雌激素和孕激素受体 (ESR1,ESR2,PGR) 是激素作用的关键介质.
研究的目的:
- 研究iSGS患者呼吸道粘膜中雌激素受体 (ESR1,ESR2) 和孕激素受体 (PGR) 的细胞特异表达.
- 为了确定受体表达是否在健康和纤维化气道组织之间有所不同.
- 为了解iSGS病变的激素驱动机制奠定基础.
主要方法:
- 利用单细胞RNA测序 (scRNAseq) 图谱,从iSGS患者的呼吸道痕中采集了25974个细胞,并与未受影响的粘膜相匹配.
- 在不同细胞子集中量化和比较ESR1,ESR2和PGR的RNA表达.
- 在纤维细胞体中使用流细胞计证实了这些受体的蛋白质表达.
主要成果:
- 在iSGS患者近接呼吸道粘膜中观察到ESR1,ESR2和PGR的差异表达.
- 在呼吸道痕组织中,纤维细胞,免疫细胞和内皮细胞显示出这些受体的显著表达.
- 纤维细胞强烈表达ESR1和PGR,免疫细胞表达ESR1和ESR2,内皮细胞主要表达ESR2.
- 在未受影响的粘膜中的上皮细胞表达了所有三种受体,但这种表达在痕组织中减少了.
结论:
- 单细胞RNA测序成功地将内分泌受体表达局部化到iSGS气道内的特定细胞类型.
- 这些发现凸显了激素信号通路在iSGS病变发生过程中的潜在作用.
- 需要进一步的研究来阐明荷尔蒙依赖机制是如何促进iSGS进展的.
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