反应性氧物种介导的内质网膜压力有助于由正统牙压力诱导的骨细胞死亡
Ming Yan1, Yun Zhang2, Wanting Niu3
1School of Automation, Hangzhou Dianzi University, Hangzhou, People's Republic of China.
Microscopy research and technique
|June 29, 2023
概括
ортодонтика力通过激活活性氧物种 (ROS) 介导的内质网膜应激 (ERS) 途径,导致骨细胞死亡. 这条通路是调节正义牙运动和预防细胞死亡的潜在目标.
科学领域:
- 生物医学工程 生物医学工程
- 细胞生物学 细胞生物学
- 矯正牙科 矯正牙科是一種矯正牙科.
背景情况:
- 骨质细胞是膜骨中的关键机械敏感细胞,对正义牙力量作出反应.
- 了解在正牙移动 (OTM) 期间骨细胞死亡机制对于优化治疗至关重要.
- 压缩力诱导的骨细胞死亡途径仍然不完全理解.
研究的目的:
- 为了研究由压力正牙力诱导的骨细胞死亡的机制.
- 探索反应性氧物种 (ROS) 介导的内质网膜应激 (ERS) 途径在这个过程中的作用.
- 识别OTM调节的潜在治疗点.
主要方法:
- 使用卷轴弹建立了一个OTM鼠标模型.
- 在实验室中对MLO-Y4骨细胞样细胞施加压力.
- 评估了细胞活力,LDH泄漏,线粒体膜潜力,ROS水平和亡标志物.
- 使用了ERS抑制剂盐和ROS清除剂N-乙-L-氨酸 (NAC).
主要成果:
- 在老鼠中,正牙力诱导了膜骨损失和骨细胞死亡.
- 在体外,压力力通过ROS介导的ERS触发了骨质细胞亡.
- 萨卢布里纳阻断了ERS诱导的亡,而NAC缓解了负载骨细胞中的ERS和亡.
结论:
- 压缩性正牙力通过ROS介导的ERS通路诱导骨细胞亡.
- 该ERS途径代表了一种新的机制,可以调节OTM期间骨细胞死亡.
- 针对ROS-ERS途径可能为控制OTM率提供新的策略.
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