在Blattodea中对adipokinetic激素及其受体进行比较分析,揭示了基因进化的新模式
Shixiong Jiang1,2, Heather G Marco3, Nina Scheich1,2
1Institute of Biology, Freie Universität Berlin, Berlin, Germany.
Insect molecular biology
|June 29, 2023
概括
阿迪波动因子激素 (AKH) 基因重复发生在Blattodea进化的早期,创造了新的. AKH受体的进化与昆虫的关系保持一致,提供了害虫控制的洞察力.
科学领域:
- 昆虫内分泌学 昆虫内分泌学
- 进化生物学是进化的生物学.
- 分子遗传学 分子遗传学
背景情况:
- 阿迪波基尼特激素 (AKH) 是一种调节昆虫新陈代谢的神经.
- AKH的功能是通过与脂肪动力激素受体 (AKHR),一种G蛋白结合受体 (GPCR) 结合.
- 了解AKH和AKHR的演变对于昆虫生理学和害虫控制至关重要.
研究的目的:
- 为了研究AKH连接体和Blattodea.的受体基因的进化史.
- 识别古老的基因复制事件和新的AKH变体.
- 探索AKH类同义词及其受体的进化起源.
主要方法:
- 分别对90种和18种的AKH前体和AKHR序列进行了家族遗传学分析.
- 分子方法和in silico方法使用转录组数据来获取受体序列.
- 对AKHR序列的比较分析,包括保存的跨膜区域和修改位点.
主要成果:
- 在Blaberoidea的共同祖先中,一个古老的AKH基因复制事件产生了新的十.
- 预测两种八和七种新型的十.
- 对AKHR序列的遗传学分析在很大程度上支持和白血统之间的确立关系.
- 在AKHR中保存了GPCR特征,单独,亚社会和社会Blattodea之间的修饰位点没有显著差异.
结论:
- 这项研究阐明了AKH和AKHR在Blattodea的进化轨迹.
- 调查结果提供了关于AKH/AKHR功能及其作为生物理性害虫控制目标对入侵性白和的潜力.
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