将高压和电场结合起来,以 Nannochloropsis oculata eicosapentaenoic 酸丰富的提取物
Sérgio Sousa1,2, Ana P Carvalho3,4, Carlos A Pinto5
1Universidade Católica Portuguesa, CBQF-Centro de Biotecnologia e Química Fina-Laboratório Associado, Escola Superior de Biotecnologia, Rua Diogo Botelho 1327, 4169-005, Porto, Portugal.
Applied microbiology and biotechnology
|June 29, 2023
概括
这项研究优化了从Nannochloropsis oculata中提取eicosapentaenoic acid (EPA) 的方法,使用高水压 (HHP) 和中等电场 (MEF) 的组合. 一种新型溶剂混合物 (乙:乙醇) 显著提高了EPA产量,为工业提供了更绿色的替代品.
科学领域:
- 生物技术是生物技术.
- 海洋生物学 海洋生物学
- 化学工程是化学工程的重要组成部分.
背景情况:
- 纳诺克洛opsis oculata是一种富含eicosapentaenoic acid (EPA) 的微藻,是一种有价值的omega-3脂肪酸.
- 高效的EPA提取对于Nannochloropsis oculata作为商业来源的经济可行性至关重要.
- 传统的提取方法往往涉及危险的溶剂,可能缺乏最佳的效率.
研究的目的:
- 为了增强eicosapentaenoic酸 (EPA) 的提取产出从Nannochloropsis oculata.
- 评估新兴技术的有效性:高水静压 (HHP) 和中等电场 (MEF).
- 研究将HHP和MEF与新型,不那么危险的溶剂混合物 (SM) 结合在一起的协同效应.
主要方法:
- 对具有不同极性指数的各种溶剂混合物 (SM) 的测试,包括一种新的二甲基乙醇:乙醇混合物.
- 高水静压 (HHP) 和中等电场 (MEF) 的单独和连续应用.
- 从湿的Nannochloropsis oculata生物质中分析总脂质和EPA产量.
主要成果:
- 与经典的福尔奇溶剂混合物 (PI 4.4) 相比,乙烯:乙醇溶剂混合物 (PI 3.6) 的每种生物质EPA显著提高 (1.3倍增加).
- 无论是HHP还是MEF单独都没有显著改善EPA提取产量.
- 顺序组合HHP和MEF处理导致EPA提取量增加62%.
结论:
- 结合HHP和MEF技术,加上优化的溶剂混合物,如乙烯:乙醇,显著增强EPA从Nannochloropsis oculata的提取.
- 这种方法为传统的提取方法提供了更环保,更有效的替代方案.
- 这些发现对寻求可持续EPA来源的食品和制药行业具有高度相关性.
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