优化和表征miRNA-129-5p封装的多 (乳糖-糖酸) 纳米颗粒来重编程激活的微质细胞
Irina Kalashnikova1, Heather Cambell1, Daniel Kolpek1
1Department of Pharmaceutical Sciences, College of Pharmacy, University of Kentucky 789 S. Limestone Lexington KY 40506 USA jonghyuck.park@uky.edu +1-859-257-1850.
Nanoscale advances
|June 29, 2023
概括
提供微RNA-129-5p的可生物降解纳米粒子调节微质激活,促进中枢神经系统再生. 这些基于PLGA的纳米配方显示出治疗神经炎症疾病的前景.
科学领域:
- 神经科学是一个神经科学.
- 生物材料科学 生物材料科学
- 免疫学 免疫学 免疫学
背景情况:
- 微质是中枢神经系统 (CNS) 炎症疾病的关键治疗点.
- 微RNAs (miRNAs) 调节免疫反应,其中miRNA-129-5p对于微质激活至关重要.
- 基于多 (乳糖-co-甘油酸) (PLGA) 的纳米粒子 (NP) 可以调节先天免疫细胞并减少神经炎症.
研究的目的:
- 优化和描述基于PLGA的NP,用于传递miRNA-129-5p.p.
- 为了研究激活微细胞调节的纳米配方的协同免疫调节效应.
- 探索炎症衍生的中枢神经系统疾病的治疗潜力.
主要方法:
- 开发了六种纳米配方,使用PLGA NPs与各种辅助剂 (EGCG,Sp,PEI) 进行miRNA-129-5p输送.
- 使用物理化学,生物化学和分子生物学方法来表征纳米配方.
- 评估了免疫调节作用和miRNA-129-5p释放动力学.
主要成果:
- 基于PLGA的NP与精素 (PLGA-miR+Sp) 和PEI (PLGA-miR+PEI) 显示出显著的免疫调节作用.
- 这些纳米配方促进了持续的miRNA-129-5p释放.
- 激活的微质细胞被偏向于一种亲再生的表型,增强了与再生相关的因素和减少了亲炎症因素.
结论:
- 基于PLGA的NP有效地传递miRNA-129-5p用于微质细胞调节.
- PLGA NPs和miRNA-129-5p之间的协同作用提供了治疗潜力.
- 这些纳米配方在治疗中枢神经系统炎症引起的疾病方面具有前景.
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