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甲醇提取物抑制ETV6-NTRK3的酸化

Hoang Thanh Chi¹, Vo Ngoc Tram², Nguyen Trung Quan²

¹Department of Medicine and Pharmacy, Thu Dau Mot University, Thu Dau Mot, Binh Duong 820000, Vietnam.

Biomedical reports

|June 29, 2023

概括

安德罗格拉菲斯 (Andrographis paniculata) 的甲醇提取物 (MeAP) 抑制了EN融合阳性癌症细胞系中的细胞增殖. MeAP治疗也减少了EN酸化,并诱导了亡,这表明了治疗潜力.

科学领域:

在瘤学瘤学.
药理学药理学是指药理学的学科.
分子生物学分子生物学

背景情况:

ETS变异转录因子6 (ETV6) -神经营养受体氨酸激酶3 (NTRK3) (EN) 融合是特定癌症中发现的罕见遗传变异.
对EN基因融合的表达需要进一步的研究和临床数据来全面理解.
这项研究调查了Andrographis paniculata甲醇提取物 (MeAP) 对EN相关细胞系的潜力.

研究的目的:

确定MeAP对与EN相关的细胞系 (IMS-M2和BaF3/EN) 的抑制作用.
评估MeAP在这些细胞系中的作用机制.
通过比较其对对照细胞 (Vero) 的影响来评估MeAP的选择性.

主要方法:

细胞活力测试 (Trypan蓝色染色,MTT) 用于评估MeAP的抑制作用.
西方涂抹和免疫沉被用于检测EN激活和酸化.
计算了IC50值以量化MeAP的强度.

主要成果:

MeAP对IMS-M2和BaF3/EN细胞系具有显著的抑制作用,其IC50值分别为12.38±0.57μg/ml和13.06±0.49μg/ml.
细胞增殖的抑制被观察到以时间,剂量和细胞密度依赖的方式.
MeAP治疗抑制了EN融合阳性细胞中的EN酸化和诱导亡,对照Vero细胞 (109.97±4.24μg/ml) 的IC50显著更高.

结论:

MeAP对EN融合阳性细胞系表现出强大的抗癌活性.
提取物通过抑制EN酸化和促进亡来起作用.
这些发现表明MeAP作为一种潜在的治疗药物,可以治疗含有EN融合的癌症.

关键词:

安德罗格拉菲斯 (Andrographis paniculata) 是一个恐慌的动物.在 BaF3/EN 的情况下.欧洲电视台6/NTRK3 在IMS-M2中使用.这就是MeAPAP的意思.

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