来自马来西亚的Ischyja marapok (Lepidoptera: Noctuoidea: Erebidae) 的完整线粒基因组数据的表征
Marylin Miga1, Puteri Nur Syahzanani Jahari1, Sivachandran Parimannan2
1Department of Biosciences, Faculty of Science, Universiti Teknologi Malaysia, Johor Bahru, Johor 81310, Malaysia.
Data in brief
|June 29, 2023
概括
对Ischyja marapok的线粒体基因组进行了测序,揭示了其遗传构成和Erebidae家族内的进化关系. 这些数据有助于理解蝶的多样性和环境监测.
科学领域:
- 基因组学就是基因组学.
- 昆虫学 昆虫学是一门学科.
- 分子遗传学分子遗传学
背景情况:
- 埃雷贝科家族 (Erebidae) 包含了虫中描述的物种数量最多,表现出显著的变化.
- 目前对伊什基亚属的线粒体基因组数据有限,这阻碍了全面的遗传学分析.
- 来自马来西亚的一种物种Ischyja marapok代表了这个多样化的家族的一个研究不足的成员.
研究的目的:
- 来自马来西亚的Ischyja marapok的完整线粒体基因组的测序和分析.
- 提供一个有价值的数据集,以了解Ischyja属的族系和多样化.
- 建立基于环境DNA的对陆地生态系统变化的评估的参考.
主要方法:
- 使用Illumina NovaSeq 6000下一代测序技术对Ischyja marapok的整个线粒基因组进行测序.
- 线粒基因组序列的生物信息分析,包括基因识别和基因组合分析.
- 遗传学树的构建,以确定Ischyja marapok的进化关系.
主要成果:
- 伊什基亚马拉波克的完整线粒基因组长达15421bp,包含13个蛋白质编码基因,22个tRNA,2个rRNA和一个控制区域.
- 线粒基因组表现出显著的A+T偏差 (80.6%),具有特定的基础组成 (A: 39.2%,T: 41.4%,C: 11.9%,G: 7.5%).
- 遗传学分析将Ischyja marapok置于Erebinae亚科中,与Ischyja manlia密切相关,具有强大的统计支持.
结论:
- 新测序的Ischyja marapok线粒基因组为Ischyja属提供了关键的遗传数据.
- 这一数据集有助于解决Erebidae家族内遗传学上的不确定性.
- 线粒基因组序列作为未来使用环境DNA进行生态研究的宝贵资源.
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