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相关概念视频

Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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相关实验视频

Updated: Jul 25, 2025

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
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在分子分辨率上空间分辨的单细胞转基因

Hu Zeng1,2, Jiahao Huang1,2, Jingyi Ren1,2

  • 1Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

Science (New York, N.Y.)
|June 29, 2023
PubMed
概括

研究人员开发了RIBOmap,这是一种在单细胞中绘制信使RNA (mRNA) 翻译的新方法. 这项技术揭示了细胞特异性的基因表达模式和大脑中的局部翻译.

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iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution
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相关实验视频

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科学领域:

  • 分子生物学
  • 遗传学
  • 神经科学

背景情况:

  • 精确控制传递 RNA (mRNA) 的翻译对于基因调节至关重要.
  • 在空间和单细胞分辨率上研究mRNA翻译具有挑战性.

研究的目的:

  • 开发一种用于细胞转化体的现场分析的新方法.
  • 用空间和单细胞分辨率对mRNA翻译进行系统研究.

主要方法:

  • 开发了与核糖体结合的mRNA映射 (RIBOmap),是一种高度多重化的3D现场分析技术.
  • 应用RIBOmap对HeLa细胞中的981个基因和小鼠大脑组织中的5413个基因进行分析.

主要成果:

  • RIBOmap 显示了 HeLa 细胞中的功能基因模块的细胞循环依赖的转化控制和同位化.
  • 产生了119,173个小鼠大脑细胞的空间分辨率.
  • 特定的细胞类型和大脑区域特异性转化调节,包括寡细胞成熟.
  • 在完整的大脑组织中检测到广泛的局部翻译.

结论:

  • RIBOmap是研究高分辨率的强大工具.
  • 该方法为复杂组织的空间和单细胞转化调节提供了新的见解.
  • 在脑细胞中的小体细胞发育和局部翻译过程中发现了动态翻译重塑.