一种植物性黄,黄素,诱导Rhizobium meliloti结节基因的表达
概括
根排泄物向Rhizobium meliloti发出信号,以形成固定的结节. 研究人员确定氨酸是关键的信号分子,控制对共生的nodABC基因表达.
科学领域:
- 植物与微生物的相互作用
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 草 (Rhizobium meliloti) 和之间的共生关系导致固根结节.
- Rhizobium meliloti nodABC基因对于早期宿主反应至关重要,例如皮质细胞分裂和根毛.
研究的目的:
- 在早期结节发育期间调查宿主-共生体信号机制.
- 为了识别诱导nodABC基因表达的精中的特定分子.
主要方法:
- 构建nodeABC-lacZ融合以选植物排泄物中的诱导活性.
- 使用光谱技术 (UV-Vis,NMR,质谱) 隔离和阐明诱导分子的结构.
主要成果:
- 研究人员发现,胡桃精液诱导nodABC基因表达,这表明早期阶段的信号传递.
- 信号分子被确定为3',4',5,7-四基黄 (luteolin).
- 作为 nodABC 表达的诱导物,一种常见的植物代谢物 - - 丁醇被证实是诱导 nodABC 表达的诱导物.
结论:
- 氨酸在控制草-生菌共生期间的nodABC基因表达方面发挥着调节作用.
- 这一发现突出了除了防御化合物之外的黄类的新功能,它们在植物微生物相互作用中充当信号分子.
更多相关视频
20:01Single-plant, Sterile Microcosms for Nodulation and Growth of the Legume Plant Medicago truncatula with the Rhizobial Symbiont Sinorhizobium meliloti
Published on: October 1, 2013
10:58Plant Promoter Analysis: Identification and Characterization of Root Nodule Specific Promoter in the Common Bean
Published on: December 23, 2017
相关概念视频
The Roles of Bacteria and Fungi in Plant Nutrition
Plants have the impressive ability to create their own food through photosynthesis. However, plants often require assistance from organisms in the soil to acquire the nutrients they need to function correctly. Both bacteria and fungi have evolved symbiotic relationships with plants that help the species to thrive in a wide variety of environments.The collective bacteria residing in and around plant roots are termed the rhizosphere. These soil-dwelling bacterial species are incredibly diverse.
Cell Signaling in Plants
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
Microbe-Plant Interactions
Microbe-plant interactions represent a dynamic spectrum of associations shaped by intricate chemical signaling. These interactions can be neutral, beneficial, or detrimental, and profoundly influence plant physiology, growth, and ecosystem function. The plant microbiome, comprising bacteria, fungi, archaea, protists, and viruses, plays a pivotal role in mediating these effects through surface colonization, internal colonization, or systemic symbiosis.Mutualistic associations, particularly with...
