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通过旋转内流层工程技术进行分层组织生物制造.

Ian Holland1, Wenmiao Shu2, Jamie A Davies1

  • 1Deanery of Biomedical Science and the Centre for Engineering Biology, University of Edinburgh, Edinburgh, United Kingdom.

Biofabrication
|June 29, 2023
PubMed
概括

研究人员开发了旋转内部流量层工程 (RIFLE),一种新的生物制造方法. 这项技术创建了高分辨率的,分层组织结构,模仿人体组织学,用于组织工程应用.

科学领域:

  • 生物材料科学 生物材料科学
  • 组织工程是组织工程.
  • 再生医学是一种再生医学.

背景情况:

  • 模仿人类组织结构的分层组织的生物组装具有挑战性.
  • 目前的生物打印缺乏微尺度细胞宽度层的分辨率,特别是像原蛋白这样的低粘度水凝.
  • 在分层组织工程中,很难实现高细胞密度.

研究的目的:

  • 引入一种新的,低成本的生物制造技术,用于创建可调节的,多层的类似组织的结构.
  • 克服现有的生物打印方法在实现微尺度分辨率和高细胞密度方面的局限性.
  • 为了证明在经济上制造具有代表性的分层组织的潜力.

主要方法:

  • 旋转内部流层工程 (RIFLE) 使用高速旋转的管状模具.
  • 小量的细胞载体液体应用于模具的内部表面,形成薄的凝层.
  • 层厚度由旋转速度控制,使得可以逐步构建具有离散微观层的宏观尺度管.

主要成果:

  • RIFLE成功地制造了可调节的,多层的组织状结构,具有微观层.
  • 高密度的细胞层 (10^8细胞/毫升) 被模拟成异质结构.
  • 在细胞宽度 (12.5微米) 的原层中与人类光滑肌肉细胞展示了tunica媒体组合.
关键词:
原蛋白是一种原蛋白.微组织的生物制造.边缘流动的流动流量.管状组织的管状组织.血管组织 血管组织

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结论:

  • RIFLE是一种生物制造复合结构的启用技术,模仿本地分层组织的复合结构.
  • 该技术促进了离散的微观层的沉积,这对于复制组织学特征至关重要.
  • RIFLE为研究人员提供了一个具有成本效益的解决方案,用于创建多种多层组织模型.