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DOCK8对于中性粒细胞介导的皮肤S. aureus感染的清除至关重要

Hazel Wilkie¹, Maheshwor Timilshina¹, Siti Rahmayanti²

¹Division of Immunology, Boston Children's Hospital and Department of Pediatrics Harvard Medical School, Boston, MA, USA.

Clinical immunology (Orlando, Fla.)

|June 29, 2023

概括

缺少DOCK8会损害中性粒细胞的功能,导致皮肤感染时黄金葡萄球菌的清除延迟. 这种敏感性与DOCK8缺乏的小鼠中中性粒细胞存活率降低和细胞化有关.

科学领域:

免疫学免疫学免疫学
皮肤病学皮肤病学
传染性疾病传染性疾病

背景情况:

DOCK8 缺乏与对细菌性皮肤感染,特别是金黄色葡萄球菌感染的易感性增加有关.
中性粒细胞对于从皮肤中清除金黄色细菌至关重要.

研究的目的:

研究在DOCK8缺乏症患者中对金黄色葡萄球菌皮肤感染的敏感性增加的机制.
为了检查中性粒细胞在S. aureus清除中的作用,在DOCK8缺乏的小鼠模型中.

主要方法:

利用剥带的小鼠模型诱导皮肤损伤和黄金色杆菌感染.
在受感染的皮肤中评估了中性粒细胞数量,活力和功能 (细胞分裂,呼吸道爆发).
分析了中性粒细胞吸引化学基因的基因表达.

主要成果:

缺少DOCK8的小鼠在机械受伤的皮肤上表现出延迟的S. aureus清除.
与对照小鼠相比,DOCK8缺乏的小鼠感染皮肤中的中性粒细胞数量和活力显著降低.
缺少DOCK8的中性粒细胞在体外表现出对细胞死亡的敏感性增加和S. aureus的细胞分裂减少,尽管呼吸系统正常爆发.

结论:

在DOCK8缺乏症中,中性粒细胞存活率下降和缺陷的细胞分裂有助于对皮肤S. aureus感染的易感性.
这些发现强调了DOCK8在中性粒细胞介导的宿主防御皮肤病原体中的关键作用.

关键词:

在 DOCK8 缺陷中.中性粒细胞中性粒细胞.发细胞症 (phagocytosis) 是一种致死细胞的发生.黄金色的S. 黄金色的

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