结核菌菌H37Rv内酶 (Rv1023) - 主体依赖性修饰对蛋白质功能表达,特征和影响
Ajay Kumar1, Vishant Mahendra Boradia1, Apurwa Mahajan2
1Department of Biotechnology National Institute of Pharmaceutical Education and Research (NIPER), Phase X, Sector 67, SAS Nagar, Punjab, 160062, India.
Biochimie
|June 29, 2023
概括
结核菌菌菌的埃诺拉酶是什么
科学领域:
- 生物化学 生物化学
- 微生物学 微生物学
- 酶学 是一种酶学.
背景情况:
- 结核菌菌埃诺拉是一种关键的糖解酶.
- 它将糖解与TCA通路联系起来,并在入侵和生物膜中发挥作用.
- PEP 枯竭与耐药,不复制的细菌有关.
研究的目的:
- 为了表达和描述Mycobacterium结核病的表现和特征.
- 为了研究表达宿主对乙醇酶功能的影响.
- 确认埃诺拉斯在生物膜形成中的作用.
主要方法:
- 在Mtb H37Ra和大肠杆菌中表达和描述Mycobacterium结核病埃诺拉酶.
- 对翻译后修改的分析.
- 评估酶活性和生物膜的形成.
主要成果:
- 表达宿主显著影响体活动和替代功能.
- 在翻译后的修改中观察到微妙的差异.
- 埃诺酶被证实在Mycobacterium结核病生物膜形成中发挥作用.
结论:
- 对表达宿主的选择对于Mycobacterium tuberculosis enolase的功能性表征至关重要.
- 了解这些差异有助于开发新的抗菌素.
- 抑制乙醇酶是对抗Mtb生物膜的一种潜在策略.
相关概念视频
01:56Structural Protein Function
Structural proteins are a category of proteins responsible for functions ranging from cell shape and movement to providing support to major structures such as bones, cartilage, hair, and muscles. This group includes proteins such as collagen, actin, myosin, and keratin.
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity. In bones and teeth, it mineralizes to form...
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity. In bones and teeth, it mineralizes to form...
01:14Rab Proteins
Rab proteins constitute the largest family of monomeric GTPases, of which 70 members are present in humans. Rab proteins and their effectors regulate consecutive stages of vesicle transport such as vesicle transport, docking, and fusion to the correct recipient membrane.
Rab proteins switch between a cytosolic, GDP-bound inactive state and a membrane-anchored, GTP-bound active state. By themselves, Rabs show slow rates of GDP/GTP exchange and GTP hydrolysis. Thus, Rab proteins are considered...
Rab proteins switch between a cytosolic, GDP-bound inactive state and a membrane-anchored, GTP-bound active state. By themselves, Rabs show slow rates of GDP/GTP exchange and GTP hydrolysis. Thus, Rab proteins are considered...
01:26Protein Modifications in the RER
Modification of secretory and transmembrane proteins entering the rough ER begins in the ER lumen. These modifications aid in protein folding and stabilize the acquired tertiary structure. Protein modifications in the rough ER co-occur at different stages of protein folding.
Broadly, these modifications can be categorized into four main categories — glycosylation, formation of disulfide bonds, assembly of protein subunits, and specific proteolytic cleavages like removal of signal sequences.
Broadly, these modifications can be categorized into four main categories — glycosylation, formation of disulfide bonds, assembly of protein subunits, and specific proteolytic cleavages like removal of signal sequences.
01:58Mechanical Protein Function
Proteins perform many mechanical functions in a cell. These proteins can be classified into two general categories- proteins that generate mechanical forces and proteins that are subjected to mechanical forces. Proteins providing mechanical support to the structure of the cell, such as keratin, are subjected to mechanical force, whereas proteins involved in cell movement and transport of molecules across cell membranes, such as an ion pump, are examples of generating mechanical force.
01:56Structural Protein Function
Structural proteins are a category of proteins responsible for functions ranging from cell shape and movement to providing support to major structures such as bones, cartilage, hair, and muscles. This group includes proteins such as collagen, actin, myosin, and keratin.
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity. In bones and teeth, it mineralizes to form...
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity. In bones and teeth, it mineralizes to form...
01:26Bacterial Protein Maturation
Bacterial protein maturation is a tightly regulated process that ensures newly synthesized polypeptides achieve correct functional conformations. This maturation involves a series of modifications, folding events, and quality control steps, often assisted by specialized chaperone proteins.N-Terminal ModificationsThe maturation of bacterial polypeptides begins cotranslationally as the polypeptide exits the ribosome. The first amino acid, N-formylmethionine (fMet), is typically modified at the...