在奶酪乳清中生长的潜在益生菌Lacticaseibacillus paracasei ItalPN16的功能性质得到改善
Cristian Mauricio Barreto Pinilla1, Adriano Brandelli2, Fabiana Galland3
1Dairy Technology Center, Institute of Food Technology (ITAL), Campinas, São Paulo, 13070-178, Brazil.
Letters in applied microbiology
|June 29, 2023
概括
甜乳清 (SW) 和酸乳清 (AW) 有效地支持益生菌Laticaseibacillus paracasei ItalPN16的生长. 乳酪基培养物增强了诸如耐酸性和粘附性等益生菌性质,提供了低成本的生产媒介.
科学领域:
- 微生物学 微生物学
- 食品科学 食品科学 食品科学
- 生物技术是生物技术.
背景情况:
- 益生菌生产需要专门的,昂贵的培养基,以获得最佳的生存能力和功能.
- Laticaseibacillus paracasei ItalPN16是一种潜在的益生菌菌株,需要在替代生长基质中进行评估.
- 乳清是一种乳制品副产品,为微生物培养基提供了潜在的低成本替代品.
研究的目的:
- 评估普通甜乳清 (SW) 和酸乳清 (AW) 作为Laticaseibacillus paracasei ItalPN16.的培养基的适用性.
- 评估乳清基介质对L. paracasei.主要益生菌特征的影响.
- 确定乳清是否可以作为益生菌生物质生产的成本有效媒介.
主要方法:
- L. paracasei ItalPN16 在37°C的巴氏化SW和AW中培养48小时.
- 量化了细菌生长 (Log CFU/ml),并监测了糖的使用情况.
- 评估了益生菌特性,包括耐酸性 (pH 2.5,3.5),自聚,细胞疏水性,生物膜形成和Caco-2细胞粘附.
主要成果:
- SW和AW都支持了L. paracasei的强增长,达到>9 Log CFU/ml,糖消耗<50%.
- 在SW和AW中培养的细胞与在标准MRS介质中培养的细胞相比,对低pH的抵抗力更强.
- SW培养显著改善了自身聚合,生物膜形成和Caco-2细胞粘附能力.
结论:
- 消毒的甜乳清和酸乳清是L. paracasei ItalPN16种植的可行,经济高效的媒介.
- 基于乳酪的种植增强了关键的益生菌功能,包括耐酸应激和粘附.
- 特别是糖乳糖,为生产功能益生菌生物质提供了可持续和经济的基质.
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