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单细胞 Hi-C 识别了背后化增强器景观的塑料染色体构造

Nimrod Rappoport^1,2, Elad Chomsky¹, Takashi Nagano^3,4

¹Department of Computer Science and Department of Biological Regulation, Weizmann Institute of Science, Rehovot, Israel.

Nature communications

|June 29, 2023

概括

胚胎细胞发育显示出细胞周期对染色体结构的强烈影响. 虽然一些细胞迅速采用特定的形状,但大多数保持灵活的染色体状态,与多种增强剂活性相关.

科学领域:

基因组学就是基因组学.
发展生物学发展生物学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

胚胎发育需要精确的细胞增殖和分化.
平衡细胞分裂与细胞命运的决定是至关重要的,但不太了解.
染色体组织在发育过程中调节基因表达起着作用.

研究的目的:

使用单细胞Hi-C.绘制胃化后小鼠胚胎细胞中的染色体构造图.
为了将染色体结构与转录状态和细胞系相关联.
研究细胞周期,表观遗传状态和细胞命运如何影响基因组组织.

主要方法:

单细胞Hi-C技术以单细胞分辨率捕捉3D基因组架构.
集成与单细胞RNA测序数据用于转录造型.
对复制时间,TAD,分区和促进剂-增强剂相互作用的分析.

主要成果:

胚胎染色体表现出显著的细胞周期特征.
在表观遗传状态之间,复制时间,TADs,分区和促进剂-增强剂接触有所不同.
原始的红细胞表现出高度紧的结构,而外皮/半皮细胞表现出血统特定的接触.

结论:

细胞承诺允许快速获得特定的染色体构造.
大多数胚胎细胞表现出由动态增强器景观影响的塑料染色体结构.
细胞周期进展和表观遗传状态在早期发育过程中显著影响基因组组织.

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Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.

Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.

Published on: May 6, 2010

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