在年轻和老年小鼠中,用于粘膜和持久的系统性SARS-CoV-2免疫力的多成分鼻内辅助剂
Sonia Jangra1,2, Jeffrey J Landers3,4,5, Gabriel Laghlali1,2
1Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
NPJ vaccines
|June 29, 2023
概括
一种结合纳米乳液和RNA技术的新型鼻腔疫苗辅助剂显示,它在所有年龄段对长期的COVID-19免疫力充满希望,其性能优于传统的辅助剂.
科学领域:
- 免疫学 免疫学 免疫学
- 疫苗学 疫苗学 疫苗学
- 传染性疾病 传染性疾病
背景情况:
- 目前的SARS-CoV-2疫苗提供了保护,但面临着免疫力下降和新兴变异的挑战.
- 鼻腔内接种疫苗引起的粘膜免疫力优于亲肠道免疫力,可能减少传播.
- 与年龄相关的免疫反应下降会影响疫苗的疗效,特别是在老年人中.
研究的目的:
- 开发和评估一种用于增强SARS-CoV-2疫苗反应的新型鼻内辅助剂 (NE/IVT).
- 评估NE/IVT辅助剂在年轻和老年小鼠中诱导的免疫反应的持久性和范围.
- 为了比较NE/IVT辅助剂与常规肌内辅助剂 (Addavax) 的免疫性.
主要方法:
- 开发一种基于纳米乳液 (NE) 的联合辅助剂和基于RNA的RIG-I激动剂 (IVT DI).
- 使用具有SARS-CoV-2受体结合域 (RBD) 的NE/IVT辅助剂制备鼻内疫苗.
- 免疫年轻和老年小鼠,然后评估幽默,粘膜和细胞免疫反应.
主要成果:
- NE/IVT鼻腔辅助剂在年轻和老年小鼠中诱导了强大而持久的幽默,粘膜和细胞免疫反应.
- 通过NE/IVT诱导的反应在各年龄组中具有同等的大小和质量,与与与年龄相关的衰退显示的Addavax不同.
- 在所有年龄段的NE/IVT免疫动物中观察到抗原特异IFN-γ/IL-2/TNF-α T细胞反应的显著诱导.
结论:
- 理性设计的NE/IVT鼻腔辅助剂显示了针对COVID-19的改善和独立于年龄的保护潜力.
- 使用NE/IVT的粘膜疫苗接种提供了一种有希望的策略,以克服当前疫苗的局限性,包括免疫力下降和与年龄相关的疗效.
- 这种方法可以增强对SARS-CoV-2的广泛保护,并减少病毒传播.
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