植物 (Phytophthora sojae) 增加了宿主三糖的积累,以获得碳并启动感染
Xiaoguo Zhu1, Di Fang1, Die Li1
1Shanghai Key Laboratory of Plant Molecular Sciences, College of Life Sciences, Shanghai Normal University, Shanghai, China.
Nature microbiology
|June 29, 2023
概括
植物豆 (Phytophthora sojae) 使用效应剂PsAvh413提高大豆三糖的产量,使其能够获得用于感染的营养素. 增强三糖生物合成增加了根和茎腐烂的易感性.
科学领域:
- 植物病理学 植物病理学
- 微生物的病原发生.
- 分子植物微生物相互作用.
背景情况:
- 植物豆 (Phytophthora sojae) 是大豆根和茎腐烂的重要原因.
- 病原体获取营养对成功感染至关重要.
- 感染期间P. sojae获得碳的机制尚不清楚.
研究的目的:
- 调查三糖生物合成在P. sojae感染大豆中的作用.
- 为了确定参与操纵宿主碳代谢的特定效应因子.
- 确定三糖生物合成是否是疾病管理的目标.
主要方法:
- 研究了P. sojae效应物PsAvh413与大豆三-6-酸盐合成酶6 (GmTPS6) 的相互作用.
- 评估了GmTPS6在与PsAvh413.3相互作用时的酶活性.
- 分析了大豆植物中的三糖积累.
- 研究了GmTPS6过度表达和对P. sojae感染的打击的影响.
主要成果:
- P. sojae效应剂PsAvh413通过增加GmTPS6酶活性来增强大豆三糖生物合成.
- P. sojae利用宿主衍生的三糖作为感染和发展的碳来源.
- 在大豆中过度表达GmTPS6可促进P. sojae感染.
- 抑制GmTPS6可以抑制P. sojae的感染,并降低疾病的严重程度.
结论:
- 大豆中的三糖生物合成是由P. sojae通过效应器PsAvh413.3操纵的.
- 在感染期间,三糖作为P. sojae的重要碳来源.
- 大豆三糖生物合成作为P. sojae的易感因子,为抗病策略提供了潜在的目标.
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