从口服液中提取阿片类药物的导电小瓶电膜提取
Tonje Gottenberg Skaalvik1,2, Chen Zhou2,3, Elisabeth Leere Øiestad2,4
1Department of Clinical Pharmacology, St. Olav University Hospital, Professor Brochs Gate 6, 7030, Trondheim, Norway.
Analytical and bioanalytical chemistry
|June 29, 2023
概括
这项研究引入了一种新的电膜提取方法,用于同时检测口服液中的13种阿片类药物. 经过验证的技术为药物滥用分析提供了一种非侵入性的方法,实现了高准确度和精度.
科学领域:
- 法医毒理学 法医毒理学
- 分析化学 分析化学
- 药理学 药理学是指药理学的学科.
背景情况:
- 口腔液体分析对于药物滥用检测越来越重要,因为它采集的方法非侵入性.
- 同时提取具有不同物理化学性质的多种阿片类药物会带来分析挑战.
研究的目的:
- 开发和验证一种新的电膜提取方法,用于同时量化口服液中的13种阿片类药物.
- 优化膜溶剂成分,以有效提取目标分析物.
主要方法:
- 使用导电瓶和由6 - 甲基库马林,硫醇和2 - 尼托菲尼洛乙烯组成的液体膜进行电膜提取.
- 使用超高性能液体染色学-并联质谱法 (UHPLC-MS/MS) 分析提取的阿片类药物.
- 根据欧洲医疗机构指南进行验证.
主要成果:
- 同时提取13种阿片类药物 (吗啡, oxycodone,编码因等) 预测的log P值在0.7到5.0之间.
- 方法验证显示了令人满意的结果,13种化合物中有12种符合日内和日间精度和偏差极限 (±15%).
- 提取恢复率在39-104% (CV ≤23%) 之间,矩阵效应为88-103% (CV ≤5%).
结论:
- 开发的电膜提取方法在口服液中同时检测阿片类药物方面是有效的.
- 这种非侵入性方法提供可靠的定量结果,可与常规查方法相比较.
- 优化的膜溶剂成分对于提取广泛的阿片类化合物至关重要.
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