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Updated: Jul 25, 2025

Development of Targeting Induced Local Lesions IN Genomes TILLING Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis

Development of Targeting Induced Local Lesions IN Genomes TILLING Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis

Published on: July 16, 2019

高效和多功能多重复原始编辑在六倍体小麦中.

Pei Ni¹, Yidi Zhao¹, Ximeng Zhou¹

¹Frontiers Science Center for Molecular Design Breeding (MOE), Key Laboratory of Crop Heterosis and Utilization (MOE), and Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing, 100193, China.

|June 29, 2023

概括

此摘要是机器生成的。

我们使用ePPEplus提高了小麦的主要编辑效率,实现了显著的改进. 这一突破使得多重基因编辑能够在植物中堆叠多个农学特征.

关键词:

六倍体小麦是一种六倍体小麦.多重基因组编辑多重基因组编辑主要编辑主要编辑逆转录酶的使用方法

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Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

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相关实验视频

Last Updated: Jul 25, 2025

Development of Targeting Induced Local Lesions IN Genomes TILLING Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis

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Published on: July 16, 2019

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

Published on: December 11, 2020

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

Published on: July 28, 2017

科学领域:

植物生物技术植物生物技术
基因编辑技术基因编辑技术
提高作物质量提高作物质量

背景情况:

主编辑 (PE) 提供精确的DNA修改,但在植物中面临效率限制.
现有的原始编辑系统需要优化,以便在小麦等作物物种中广泛应用.

研究的目的:

开发一套改进的六合状小麦原始编辑系统,以提高效率.
建立一个多重原始编辑平台,用于在小麦中同时进行多基因编辑.

主要方法:

通过在ePPEmax架构的反转录酶中引入V223A替代,设计了一个升级的植物主要编辑器 (ePPEplus).
在六倍体小麦原塑和再生植物中测试了ePPEplus,以提高效率和复合能力.

主要成果:

ePPEplus显示了显著更高的编辑效率,比以前的系统平均增加了33.0倍和6.4倍.
实现了强大的多重原始编辑,同时编辑原生质中的4-10个基因和再生植物中的多达8个基因.
在多重编辑实验中观察到的编辑频率高达74.5%.

结论:

ePPEplus系统大大提高了六倍状小麦的初级编辑效率和能力.
开发的多重原始编辑平台是一个强大的工具,用于在小麦中堆叠多个农学特征.
这一进步扩大了主要编辑的应用范围,以加速作物育种和遗传改进.