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环-SNX27海绵式miR-375/RPN1轴有助于肝细胞癌的进展

Chao Zheng¹, Jin Liang², Shoude Yu²

¹Surgical Department, Wuhan Hospital of Traditional Chinese Medicine, Wuhan 430000, China.

The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology

|June 30, 2023

概括

循环RNA SNX27 (circ-SNX27) 通过通过海绵化miR-375.5通过升调ribophorin I (RPN1) 来促进肝细胞癌 (HCC) 的进展. 向circ-SNX27可能为HCC治疗提供一种新的治疗策略.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
生物化学生物化学

背景情况:

肝细胞癌 (HCC) 是一个主要的全球健康问题,死亡率高.
循环RNAs (circRNAs) 在HCC病变发生中的作用是一个新兴的研究领域.
特定的circRNAs,如circ-SNX27,尚未完全研究它们对HCC进展的参与.

研究的目的:

为了阐明circ-SNX27在肝细胞癌 (HCC) 进展中的精确作用.
研究 circ-SNX27 影响 HCC.的潜在分子机制.
评估circ-SNX27作为HCC的治疗点的潜力.

主要方法:

量化实时PCR和西式斑点测试用于测量HCC细胞系和患者样本中的circ-SNX27,miR-375和RPN1表达.
进行了功能性测试,包括细胞入侵,增殖 (CCK-8) 和 caspase-3 活性.
用路西法酶记者测定,RNA免疫沉降 (RIP) 和异种移植小鼠模型来确认分子相互作用和影响.

主要成果:

在HCC组织和细胞中,Circ-SNX27和RPN1的表达升高,而miR-375的表达降低.
抑制circ-SNX27抑制了HCC细胞的增殖和入侵,增加了caspase-3活性,并抑制了瘤生长in vivo.
Circ-SNX27作为miR-375的分子海绵,从而调节RPN1表达并促进HCC恶性病变.

结论:

通过调节miR-375/RPN1轴,Circ-SNX27显著加速肝细胞癌的进展.
这些发现突出了circ-SNX27作为潜在的诊断生物标志物和HCC的治疗点.
准circ-SNX27/miR-375/RPN1通路为HCC治疗提供了一个有希望的策略.

关键词:

肝细胞癌是肝细胞癌.核武器的扩散泡活动活动.环RNA 环RNA 是一个环RNA.这是一个小RNARNA.

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