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在小鼠中因烧伤引起的单细胞中分化表达的miRNAs

Hong-Sheng Liu¹, Lun-Chao Li¹, Man Wang¹

¹Department of Emergency, Fourth Medical Center of Chinese PLA General Hospital, Beijing, People's Republic of China.

International wound journal

|June 30, 2023

概括

烧伤损伤会改变脏单细胞中的microRNA (miRNA) 表达,影响免疫反应. 像miR-146a和miR-3091-6p这样的特定miRNA显示差异调节,可能准关键的免疫信号通路.

科学领域:

免疫学免疫学免疫学
分子生物学分子生物学
生物标志物发现发现

背景情况:

烧伤严重影响免疫系统,特别是单细胞.
微RNA (miRNA) 是基因表达和免疫反应的关键调节者.
在单细胞中识别miRNA生物标志物可以提供有关烧伤病理生理学的见解.

研究的目的:

在受烧伤小鼠的单细胞中识别差异表达的miRNA及其潜在标.
调查这些miRNAs在调节单细胞功能和先天性免疫反应后烧伤中的作用.

主要方法:

雄性Balb/c小鼠经历了假冒或烧伤 (15%的TBSA).
脏CD11b+单细胞被分离和培养.
分析了单细胞增殖 (MTT试验) 和细胞因子分泌 (ELISA).
使用miRNA微阵列进行miRNA表达概况分析,并通过qRT-PCR进行验证.
生物信息工具 (Miranda,TargetScan) 预测了miRNA的目标.

主要成果:

单细胞活性在两组之间是可比的,但受烧伤的单细胞分泌的TNF-α和TGF-β含量较高,MCP-1含量较低.
在受烧伤的小鼠单细胞中,有54个miRNA被差异表达.
在燃烧后,miR-146a显著下调,而miR-3091-6p在燃烧后显著上调.
对miR-146a的预测目标包括TRAF6,IRAK1;对于miR-3091-6p,包括SOCS7,ARRB2.

结论:

烧伤会导致脏单细胞miRNA表达的显著变化.
失调的miRNAs,如miR-146a和miR-3091-6p,可能在调节对烧伤的先天免疫反应中发挥作用.
这些miRNA及其点代表着潜在的烧伤伤害生物标志物.

关键词:

CD11b+磁性珠子 CD11b+磁性珠子 CD11b+磁性珠子 CD11b+磁性珠子烧伤伤害伤害伤害的伤害.这就是miRNAs.微阵列是微阵列中的一个.一个单细胞单细胞.

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相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...