转录和蛋白质组分析.
Liu Xiao-Jing1, Xing Bao1, Wang Meng-Yu1
1Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, MARA Key Laboratory on Safety Assessment (Molecular) of Agri-GMO, Beijing, China.
GM crops & food
|June 30, 2023
概括
使用CRISPR-Cas9和腺基编辑器 (ABE) 在大米中进行基因编辑显示了最小的意外效应. 奥米克斯分析没有发现新的基因或蛋白质,这表明基因编辑作物的安全性.
科学领域:
- 农业科学 农业科学
- 分子生物学分子生物学
- 生物技术是生物技术.
背景情况:
- 基因编辑技术,如CRISPR-Cas9和腺基编辑器 (ABE) 提供精确的作物修改.
- 评估基因编辑的意外影响对于作物安全和监管批准至关重要.
- 奥米克斯方法为生物系统提供了全面的分子洞察力.
研究的目的:
- 评估CRISPR-Cas9和ABE基因编辑在大米中的意外分子后果.
- 为了比较基因编辑大米的转录组和蛋白组资料与野生类型的Nipponbare.
- 为了确定基因编辑是否引入新的基因或蛋白质,影响作物安全.
主要方法:
- 对CRISPR-Cas9和ABE编辑的大米和野生类型的Nipponbare进行了转录组分析 (RNA测序).
- 蛋白质组学分析 (质谱) 在相同的水样本上进行.
- 基因和基因组的京都百科全书 (KEGG) 路径丰富分析用于解释差异表达的基因和蛋白质.
主要成果:
- 转录组分析分别在Cas9/Nip和ABE/Nip比较中发现了520个和566个差异表达基因 (DEG).
- 蛋白质组学分析在Cas9/Nip和ABE/Nip比较中分别发现了298个和54个差异表达蛋白 (DEP).
- 综合分析显示,基因编辑大米中没有新生成的转录或蛋白质,对整体转录水平的影响最小.
结论:
- 在大米中的CRISPR-Cas9和ABE基因编辑对转录组和蛋白质组产生了有限的意外影响.
- 确定的DEG主要涉及环境适应途径,如植物病原体相互作用和信号转导.
- 在本研究中分析的基因编辑大米系被认为是安全的,因为没有检测到新的基因或蛋白质.
相关概念视频
02:24Ribosome Profiling
3.6K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.6K
01:33Proteomics
7.5K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
7.5K