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两个cystatin C试剂和四个方程对标准化后的淋巴膜过率估计的影响
Hyun-Seung Lee1, Go-Eun Bae2, Jung Eun Lee3
1Department of Laboratory Medicine, School of Medicine, Wonkwang University, Iksan, Korea.
Annals of laboratory medicine
|June 30, 2023
概括
血清囊素C (cysC) 的测量显示出变异性,特别是在低度时,影响估计的淋巴细胞过率 (eGFR) 计算. 不同的试剂和方程组合产生不一致的eGFR结果,突出了标准化挑战.
科学领域:
- 临床化学 临床化学
- 生物标记分析 生物标记分析
- 功能评估 功能评估
背景情况:
- 血清囊素C (cysC) 是估计膜过率 (eGFR) 的可靠生物标志物,与肌素相比,性别和肌肉质量等因素的影响较小.
- 尽管有可用的认证参考材料,但CysC测量的标准化仍面临挑战.
- 结合不同的CysC试剂和eGFR方程的影响仍然不清楚.
研究的目的:
- 为了评估两个标准化试剂 (Gentiancys和Rochecys) 之间的CysC测量的一致性.
- 通过将这些cysC测量与四个不同的方程 (CKD-EPIcys,CAPAeq,FASeq,EKFCcys) 结合起来,来评估生成的eGFR估计的可变性.
主要方法:
- 在使用Gentiancys和Rochecys试剂对148名参与者的cysC测量进行了模拟分析.
- 计算eGFR的方法是使用两个CysC测量结果和四个不同的方程的八种组合.
- 统计分析包括回归和林的一致性相关系数来评估一致性.
主要成果:
- Gentiancys (1.72±1.44 mg/L) 和Rochecys (1.71±1.35 mg/L) 之间的平均囊水平是可以比较的.
- 在0.85-4.40 mg/L范围内观察到反应剂一致性,总允许误差为±7.61%.
- 林对eGFR的一致性相关系数因试剂-方程组合而有显著差异 (0.73至1.00).
结论:
- 在低度 (<0.85 mg/L) 时,两种试剂之间的 cysC 值的等价性是不令人满意的.
- 不同的测量系统和方程组合可能会导致计算的eGFR有很大差异.
- 这些发现强调了需要在CysC测定中改进标准化,以准确地确定eGFR.
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