相关实验视频
Updated: Jul 25, 2025

08:05
Staining the Cytoplasmic Ca2+ with Fluo-4/AM in Apple Pulp
Published on: November 6, 2021
4.5K
MdNAC104通过CBF依赖和CBF独立的途径积极调节果的寒冷耐受性
Chuang Mei1,2, Jie Yang1, Quanlin Mei1
1State Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of Apple, College of Horticulture, Northwest A & F University, Yangling, Shaanxi, China.
Plant biotechnology journal
|June 30, 2023
概括
NAC转录因子MdNAC104通过调节花素和抗氧化剂基因,增强了果的耐寒性. 这项研究揭示了MdNAC1044.
科学领域:
- 植物科学 植物科学
- 分子生物学分子生物学
- 农业科学 农业科学
背景情况:
- 低温显著影响作物产量和分布,阻碍了水果产业.
- 众所周知,NAC转录因子 (TF) 家族调节植物耐寒性,但具体机制尚不清楚.
研究的目的:
- 阐明NAC TF MdNAC104在果寒冷耐受性中的作用和调节机制.
主要方法:
- 转基因植物中MdNAC104的过度表达.
- 在寒冷压力下分析生理参数 (离子泄漏,ROS,氧化物,抗氧化酶).
- 转录组,代谢组和促进体结合分析.
主要成果:
- 过度表达MdNAC104提高了耐寒性,减少了离子泄漏和反应性氧物种 (ROS),同时增加了氧化物和抗氧化酶活性.
- MdNAC104直接针对MdCBF1和MdCBF3促进体,增强它们的表达.
- 在寒冷压力下,MdNAC104可提高抗氧化酶基因 (例如MdCHS-b,MdANS-b) 和抗氧化酶基因 (例如MdFSD2,MdPRXR1.1) 的调节.
结论:
- MdNAC104在果寒冷耐受性方面发挥着积极作用.
- 监管机制涉及CBF依赖和CBF独立的路径.
- MdNAC104在对寒冷的反应中影响了安托西亚宁积累和抗氧化剂防御系统.
相关概念视频
Responses to Heat and Cold Stress
13.6K
Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.
13.6K
Adaptations that Reduce Water Loss
25.8K
Though evaporation from plant leaves drives transpiration, it also results in loss of water. Because water is critical for photosynthetic reactions and other cellular processes, evolutionary pressures on plants in different environments have driven the acquisition of adaptations that reduce water loss.
25.8K
C4 Pathway and CAM
45.7K
Most plants use the C3 pathway for carbon fixation. However, some plants, such as sugar cane, corn, and cacti that grow in hot conditions, use alternative pathways to fix carbon and conserve energy loss due to photorespiration. Photorespiration is the process that occurs when the oxygen concentration is high. Under such conditions, the rubisco enzyme in the Calvin cycle binds O2 instead of CO2, which halts photosynthesis and consumes energy.
C4 Pathway
The C4 pathway is used by plants such as...
C4 Pathway
The C4 pathway is used by plants such as...
45.7K
Global Regulatory Systems
44
Global regulatory systems in bacteria enable rapid and coordinated responses to environmental changes by integrating sensory inputs with gene expression, ensuring efficient adaptation to fluctuating conditions. Key global regulatory mechanisms include regulons, two-component systems, sigma factors, and secondary messengers.Regulons and Global RegulatorsA regulon is a collection of genes and operons controlled by a common global regulator. These regulators enable bacteria to prioritize resource...
44
Regulation of Transpiration by Stomata
28.5K
During photosynthesis, plants acquire the necessary carbon dioxide and release the produced oxygen back into the atmosphere. Openings in the epidermis of plant leaves is the site of this exchange of gasses. A single opening is called a stoma—derived from the Greek word for “mouth.” Stomata open and close in response to a variety of environmental cues.
28.5K
Gene Regulation During Sporulation
36
Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...
36

