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从索马到突触:成像与年龄相关的棒光受体变化在鼠标与可见光OCT可见光

Pooja Chauhan¹, Aaron M Kho², Vivek J Srinivasan^1,2,3

¹Department of Radiology, NYU Langone Health, New York, New York.

Ophthalmology science

|June 30, 2023

概括

可见光OCT揭示了老年相关的老鼠视网膜光受体的变化. 它描绘了外核层条纹和外状层子带,与细胞和突触变化相关联.

科学领域:

眼科医生眼科眼科
神经科学是一个神经科学.
生物医学成像技术生物医学成像技术

背景情况:

接近红外的OCT显示了外核层 (ONL) 和外形层 (OPL) 作为单一的波段.
在ONL和OPL复杂的内部组织不能通过标准的OCT解决.

研究的目的:

用可见光在小鼠视网膜中的OCT来图像和解释光感受器特征中与年龄相关的亚层变化.
以比以前可能更高的分辨率调查ONL和OPL与年龄相关的变化.

主要方法:

使用1.0μm轴分辨率可见光光谱/富里埃域OCT系统进行体内视网膜成像.
进行了ex vivo光和电子显微镜以进行组织学相关性.
使用线性混合效应模型或回归来进行统计分析.

主要成果:

可见光OCT揭示了ONL中的反射振荡 (条纹),与光受体核的排列相对应.
在OPL中确定了一种适度反射的子频段,与杆球体相关.
与年龄相关的ONL条纹的压缩和OPL子带的稀薄表明 soma组织和突触缩小的变化.

结论:

可见光OCT成像小鼠的OPL解决了后突触和突触差异.
这种技术允许研究杆光受体从 soma 到 synapse 的变化 in vivo.
可见光OCT为评估细胞水平上与年龄相关的视网膜变化提供了一种新方法.

关键词:

衰老的衰老衰老的衰老光学连贯性断层扫描技术.摄影受体是一种光学受体.棒子棒子棒子突触 (Synapses) 是一个突触的组成部分.

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