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一个单细胞分辨率的人类视网膜的多omics地图集

Qingnan Liang^1,2, Xuesen Cheng¹, Jun Wang¹

¹HGSC, Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA.

|June 30, 2023

概括

这项研究呈现了人类视网膜的综合多组图谱,揭示了细胞异质性和跨物种的基因调节. 这些发现为了解单细胞分辨率下视网膜分子特征提供了宝贵的资源.

科学领域:

基因组学和分子生物学
神经科学是一个神经科学.
眼科医生眼科眼科

背景情况:

人类视网膜细胞种群在丰富度上表现出显著的异质性.
了解细胞类型特定的分子机制对于视网膜研究至关重要.

研究的目的:

创建一个成年人视网膜的综合多组合单细胞地图.
描述人类视网膜内的细胞异质性和基因调节.
为了能够进行视网膜细胞类型和调控元素的跨物种比较.

主要方法:

从超过250,000个和137,000个核中分别生成和集成的单核RNA测序 (snRNA-seq) 和单核ATAC测序 (snATAC-seq) 数据.
进行了人类,子,老鼠和的视网膜图谱的跨物种比较分析.
进行了整合性分析,以确定cis元素-基因对和转录因子 (TF) 点规律.

主要成果:

在物种中识别了保存和非保存的细胞类型.
与动物和肉视网膜相比,观察到灵长类视网膜的整体细胞异质性下降.
发现了35,000个遥远的cis元素基因对,并为200多个TFs构建了调节子.
在cis元素-基因关系中揭示了细胞类型特定的异质性.

结论:

介绍了人类视网膜的综合单细胞多组图谱.
在单细胞分辨率下提供系统分子表征的资源.
突出了视网膜细胞类型的保存和分化的特征以及物种间的基因调节.

关键词:

跨物种分析跨物种分析基因调节基因调节基因调节人类视网膜人类视网膜一个单细胞多细胞的奥米克.

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