Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

7.1K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
7.1K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

13.4K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
13.4K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Associations of age, health literacy, BMI, tobacco use, and COVID-19 infection history with sexual dysfunction among COVID-19 survivors: A multicenter study.

Public health·2026
Same author

Quality of Life of Vietnamese Ostomy Patients: A Multidimensional Assessment Using the City of Hope Quality of Life-Ostomy Questionnaire (CoH-QoL-OQ).

Cureus·2026
Same author

Comparative Outcomes and Management of Vesicourethral Anastomotic Stenosis After Contemporary Standard and Pelvic-Fascia-Sparing Robotic-Assisted Radical Prostatectomy Techniques.

JU open plus·2026
Same author

Robotic buccal mucosal graft ureteroplasty: a contemporary multi-institutional experience.

World journal of urology·2026
Same author

Robotic-assisted ureteral reconstruction in renal transplant recipients: a multi-Institutional review of outcomes and complications.

World journal of urology·2026
Same author

Single Saliva Sample Linezolid Dosing for Multidrug-Resistant Tuberculosis: A Population Pharmacokinetic Modelling of Plasma and Saliva.

Clinical pharmacokinetics·2026

相关实验视频

Updated: Jul 25, 2025

Compact Quantum Dots for Single-molecule Imaging
17:14

Compact Quantum Dots for Single-molecule Imaging

Published on: October 9, 2012

18.2K

紧的,快速闪的无CD量子点,用于超分辨率光成像.

Anh T Nguyen1, Dustin R Baucom1, Yong Wang2

  • 1Department of Chemistry and Biochemistry, University of Arkansas, 345 North Campus Drive, Fayetteville, Arkansas 72701, United States.

Chemical & biomedical imaging
|June 30, 2023
PubMed
概括

紧,无的量子点 (QD) 为超分辨率成像提供了一个有前途的替代方案. 这些CuInS2/ZnS QD提供了与有毒CdSe QD相比的分辨率,但在生物应用中更小,更安全.

更多相关视频

Production and Targeting of Monovalent Quantum Dots
10:16

Production and Targeting of Monovalent Quantum Dots

Published on: October 23, 2014

25.6K
Resonance Fluorescence of an InGaAs Quantum Dot in a Planar Cavity Using Orthogonal Excitation and Detection
12:57

Resonance Fluorescence of an InGaAs Quantum Dot in a Planar Cavity Using Orthogonal Excitation and Detection

Published on: October 13, 2017

9.2K

相关实验视频

Last Updated: Jul 25, 2025

Compact Quantum Dots for Single-molecule Imaging
17:14

Compact Quantum Dots for Single-molecule Imaging

Published on: October 9, 2012

18.2K
Production and Targeting of Monovalent Quantum Dots
10:16

Production and Targeting of Monovalent Quantum Dots

Published on: October 23, 2014

25.6K
Resonance Fluorescence of an InGaAs Quantum Dot in a Planar Cavity Using Orthogonal Excitation and Detection
12:57

Resonance Fluorescence of an InGaAs Quantum Dot in a Planar Cavity Using Orthogonal Excitation and Detection

Published on: October 13, 2017

9.2K

科学领域:

  • 纳米技术纳米技术
  • 生物物理学的生物物理.
  • 显微镜的使用方法

背景情况:

  • 量子点 (QD) 是超高分辨率成像的重要光探针,使可视化超出衍射极限.
  • 基于的QD (例如CdSe) 面临毒性和大尺寸 (10-20 nm) 的限制,阻碍了生物应用.
  • 开发更小,无毒的替代品对于推进单分子局部化显微镜 (SMLM) 是必不可少的.

研究的目的:

  • 为了评估紧 (4-6纳米) 无CuInS2/ZnS (CIS/ZnS) 量子点作为光探针.
  • 将CIS/ZnS QD与商业CdSe/ZnS QD在超高分辨率成像中的性能进行比较.
  • 评估QD类型的眼行为,定位精度和成像分辨率.

主要方法:

  • 紧的CIS/ZnS QDs的合成和表征.
  • 在CIS/ZnS和CdSe/ZnS QD之间对闪动态 (启/关时间) 的比较分析.
  • 通过单分子局部化显微镜 (SMLM) 使用QD两种类型的行为丝超分辨率成像.
  • 与传统的全内部反射光显微镜 (TIRF) 相比,对分辨率改善的定量评估.

主要成果:

  • 无论是CIS/ZnS还是CdSe/ZnS QD,其成像分辨率都比传统的TIRF显微镜提高了4.5-5.0倍.
  • 尽管亮度较低,但CIS/ZnS QD的开机时间较短,关机时间较长,减少了标签重叠.
  • 与较大的CdSe/ZnS QD相比,CIS/ZnS QD的紧尺寸 (4-6 nm) 对生物标记具有优势.

结论:

  • 无CIS/ZnS QD是超高分辨率成像中CdSe/ZnS QD的一个可行的替代品.
  • 对于生物应用,CIS/ZnS QDs提供了可比的分辨率,并改善了安全性和尺寸配置.
  • 这些发现支持使用CIS/ZnS QD来推进单分子超分辨率成像技术.