Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Transposons01:24

Transposons

65
Transposons, or "jumping genes," are small mobile genetic elements (MGEs) that range from 700 to 40,000 base pairs in length. They are found in all organisms and can move within the same chromosome or transfer to different chromosomes. In some cases, transposons can also jump between different host DNA molecules, such as plasmids or viruses, contributing to genetic variability.Barbara McClintock first discovered these mobile genetic elements in the 1940s while studying maize genetics, and she...
65

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Automated workflow for genotyping individual transposon library variants.

BMC methods·2026
Same author

Construction of a syntrophic <i>Pseudomonas putida</i> consortium with reciprocal substrate processing.

Synthetic biology (Oxford, England)·2025
Same author

Standardized Quorum Sensing Tools for Gram-Negative Bacteria.

ACS synthetic biology·2025
Same author

Workflow to Select Functional Promoter DNA Baits and Screen Arrayed Gene Libraries in Yeast.

Current protocols·2024
Same author

The Laboratory Automation Protocol (LAP) Format and Repository: A Platform for Enhancing Workflow Efficiency in Synthetic Biology.

ACS synthetic biology·2023
Same author

GENETTA: a Network-Based Tool for the Analysis of Complex Genetic Designs.

ACS synthetic biology·2023

相关实验视频

Updated: Jul 25, 2025

Generation and Multi-phenotypic High-content Screening of Coxiella burnetii Transposon Mutants
11:44

Generation and Multi-phenotypic High-content Screening of Coxiella burnetii Transposon Mutants

Published on: May 13, 2015

10.0K

pBLAM1-x:用于高通量查的标准化转子子工具.

Lorea Alejaldre1, Ana-Mariya Anhel1, Ángel Goñi-Moreno1

  • 1Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid (UPM)-Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA/CSIC), Madrid, Spain.

Synthetic biology (Oxford, England)
|June 30, 2023
PubMed
概括

研究人员开发了一种新的遗传工具,pBLAM1-x等离子体载体,以简化合成生物学应用的高通量转子子体插入测序 (TnSeq). 该工具增强了遗传结构的表征,并帮助数学建模,以改进设计-建造-测试周期.

科学领域:

  • 合成生物学 合成生物学
  • 分子生物学分子生物学
  • 基因组学就是基因组学.

背景情况:

  • 工程细胞功能需要精确的遗传工具合成生物学.
  • 鉴定遗传构造的特征需要对数学建模和设计-构建-测试周期进行细致的测量.

研究的目的:

  • 开发和展示一种新的遗传工具,pBLAM1-x等离子体载体,旨在促进高通量转子子体插入测序 (TnSeq).
  • 为了证明pBLAM1-x工具在分析细菌*Pseudomonas putida*内的遗传结构中的实用性.

主要方法:

  • 开发基于迷你Tn5转位子向量pBAMD1-2的pBLAM1-x等离子体向量,并遵循欧洲标准向量架构 (SEVA) 格式.
  • 将Himar1 Mariner转化酶系统纳入pBLAM1-x向量中.
  • 分析了来自60个*Pseudomonas putida* KT2440克隆的测序数据,以评估该工具的性能.

主要成果:

  • 成功开发了pBLAM1-x等离子体载体,这是一种用于高通量TnSeq.的新型遗传工具.
  • 证明了pBLAM1-x工具在分析*Pseudomonas putida*中的转子体插入时的表现.
  • 将pBLAM1-x工具集成到SEVA数据库中,强调其标准化和可访问性.
关键词:
染色体插入发生在染色体内.遗传工具是一种遗传工具.这是一种等离子体.标准格式标准格式.转换的意思就是转换.

更多相关视频

Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing
08:19

Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing

Published on: July 7, 2020

10.5K
An Efficient In Vitro Transposition Method by a Transcriptionally Regulated Sleeping Beauty System Packaged into an Integration Defective Lentiviral Vector
10:13

An Efficient In Vitro Transposition Method by a Transcriptionally Regulated Sleeping Beauty System Packaged into an Integration Defective Lentiviral Vector

Published on: January 12, 2018

9.0K

相关实验视频

Last Updated: Jul 25, 2025

Generation and Multi-phenotypic High-content Screening of Coxiella burnetii Transposon Mutants
11:44

Generation and Multi-phenotypic High-content Screening of Coxiella burnetii Transposon Mutants

Published on: May 13, 2015

10.0K
Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing
08:19

Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing

Published on: July 7, 2020

10.5K
An Efficient In Vitro Transposition Method by a Transcriptionally Regulated Sleeping Beauty System Packaged into an Integration Defective Lentiviral Vector
10:13

An Efficient In Vitro Transposition Method by a Transcriptionally Regulated Sleeping Beauty System Packaged into an Integration Defective Lentiviral Vector

Published on: January 12, 2018

9.0K

结论:

  • pBLAM1-x等离子体载体代表了高通量TnSeq的重大进步,简化了合成生物学中的基因工程和表征.
  • 该工具支持设计-构建-测试生命周期,通过使数学建模的有效数据采集成为可能.
  • 将其纳入SEVA数据库可以确保在微生物研究和合成生物学中得到更广泛的采用和应用.