一种以前未被描述的与分裂体相关的脂蛋白,DalaA,是Rhodobacterales中正常细胞分裂所需要的
François Alberge1, Bryan D Lakey1,2, Ryan E Schaub3
11 Wisconsin Energy Institute, Great Lakes Bioenergy Research Center, University of Wisconsin-Madison , Madison, Wisconsin, USA.
mBio
|June 30, 2023
概括
研究人员确定了DalaA,这是Rhodobacter sphaeroides中一种新的外膜脂蛋白,对细菌细胞分裂至关重要. 达拉A的损失会破坏细胞分裂和抗生素敏感性,突出其在分裂中的作用.
科学领域:
- 细菌细胞外生物发生和功能.
- 格拉姆阴性细菌的细胞分裂机制.
- 微生物系统中的蛋白质与蛋白质相互作用.
背景情况:
- 细菌细胞外对于生存至关重要,影响抗生素耐药性,营养吸收和细胞形状.
- 了解参与细胞外动态的蛋白质对于破译细菌生理学至关重要.
- 阿尔法蛋白质细菌拥有独特的细胞外组成部分,需要进行研究.
研究的目的:
- 描述Rhodobacter sphaeroides中未被描述的蛋白质RSP_1200的功能.
- 研究RSP_1200在细菌细胞分裂和细胞外完整性中的作用.
- 在Alphaproteobacteria中识别细菌分裂体的新组成部分.
主要方法:
- 光蛋白标记和活细胞成像用于跟踪细胞周期期间RSP_1200定位.
- 同免疫沉测试以确定相互作用的蛋白质,包括FtsZ和Pal.
- 基因操纵 (基因删除) 来评估RSP_1200损失的表型后果.
- 抗生素敏感性测试用于评估对细胞包膜合成的影响.
主要成果:
- RSP_1200被确定为一种外膜脂蛋白,可以结合peptidoglycan.
- RSP_1200在分裂过程中动态定位到细胞隔膜,反映FtsZ环位置.
- 删除RSP_1200 (∆RSP_1200) 导致细胞分裂缺陷,抗生素敏感性变化和外膜突起.
- 建议RSP_1200是一个新的分裂组分,名为DALA (分裂相关脂蛋白A).
结论:
- 达拉A是一种新发现的Rhodobacter sphaeroides分裂体的组成部分,对适当的细胞分裂至关重要.
- 达尔A可能会作为支架,在分裂部位定位或调节甘氨酸转酶.
- 这些发现为控制格兰阴性细菌,特别是Alphaproteobacteria细胞分裂的宏分子机制提供了新的见解.
- 其他罗多细菌体中存在 DalA 同类物,这表明它在细胞分裂中起着保留作用.
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