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相关概念视频

RNA-seq03:21

RNA-seq

10.1K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
10.1K
Nucleic Acid Structure01:25

Nucleic Acid Structure

6.2K
The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA...
6.2K
MicroRNAs01:22

MicroRNAs

21.4K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
21.4K

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相关实验视频

Updated: Jul 25, 2025

Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism
11:37

Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism

Published on: July 28, 2017

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微RNA池合成使用并联固相寡核酸合成.

Sofie Slott1, Kira Astakhova1

  • 1Department of Chemistry, Technical University of Denmark, 206-207, 2800 Kgs. Lyngby, Denmark.

The Journal of organic chemistry
|June 30, 2023
PubMed
概括

研究人员开发了一种新的方法来合成针对乳腺癌细胞的微RNA池. 这种Tandem Oligonucleotide合成策略有效地产生高产量的microRNA池,用于研究和技术应用.

科学领域:

  • 生物化学 生物化学
  • 分子生物学分子生物学
  • 合成生物学 合成生物学

背景情况:

  • 微RNAs (miRNAs) 是基因表达的关键调节者.
  • 针对性的miRNA合成对于研究和治疗应用至关重要.
  • 现有的生产miRNA池的方法可能是低效的.

研究的目的:

  • 开发一种新的,高效的方法来合成微RNAs池.
  • 为了创建针对乳腺癌细胞的微RNA池.
  • 研究微RNA池合成中提高产品产量的策略.

主要方法:

  • 使用了Tandem Oligonucleotide合成策略,在单个固体支上同时合成多个miRNA.
  • 采用2'/3'OAc核酸胺,以创建一个可切割的部分,用于分离单个miRNAs.
  • 研究了线性和分支 (dendrimer) 池配置以优化产量.

主要成果:

  • 成功合成了多达四个连续的miRNAs (miR129-1-5p,miR31,miR206和miR27b-3p) 的池,总长度为88个核酸.
  • 开发了胺酸,使合成后裂变能够释放单个miRNAs.
  • 合成的微RNA池的高产量得到证明.

更多相关视频

A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA
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A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA

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Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing
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Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing

Published on: November 18, 2014

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相关实验视频

Last Updated: Jul 25, 2025

Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism
11:37

Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism

Published on: July 28, 2017

19.1K
A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA
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A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA

Published on: December 2, 2009

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Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing
14:15

Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing

Published on: November 18, 2014

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  • 在线性和分支的微RNA树枝体池之间比较产品产量.

    • 结论:

    • 双重寡核酸合成策略提供了一种高效的方法,用于生产高产量的microRNA池.
    • 该方法解决了在研究和技术中对合成RNA寡合物的日益增长的需求.
    • 开发的技术适用于为包括癌症研究在内的各种应用创建定制的microRNA池.