两种测定方法的比较,用于诊断中枢神经系统感染患者的简单疹病毒

相关实验视频

Updated: Jul 31, 2026

A Primary Neuron Culture System for the Study of Herpes Simplex Virus Latency and Reactivation

A Primary Neuron Culture System for the Study of Herpes Simplex Virus Latency and Reactivation

Published on: April 2, 2012

J T Gaensbauer¹, E C Fernholz², L M Hiskey¹

¹Department of Pediatric and Adolescent Medicine, Division of Pediatric Infectious Diseases, USA.

Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology

|June 30, 2023

概括

多重PCR面板对简单疹病毒 (HSV) 检测的敏感性低于向PCR. 不一致的结果表明较轻的HSV疾病,指导适当的测试选择.

科学领域:

临床微生物学临床微生物学
传染性疾病传染性疾病
分子诊断学分子诊断学

背景情况:

多重PCR面板被广泛用于诊断中枢神经系统感染.
这些面板对于检测单纯疹病毒 (HSV) 的灵敏度较低,与向PCR测定相比.
同时使用多重 (BioFire MEP) 和向HSVPCR (Diasorin Simplexa) 允许进行测定比较和患者表征.

研究的目的:

在临床环境中比较多重和向HSV PCR试验的性能.
鉴定两种HSV PCR方法之间具有一致的阳性或不一致的结果的患者.
为了为临床决策提供有关多重和向HSVPCR的同时使用的信息.

主要方法:

在2018年10月12日至2022年6月6日期间,对1387名患者进行了追溯分析,同时使用多重检测 (BioFire MEP) 和向HSVPCR (Diasorin Simplexa) 进行了测试.
患者之间实验室,诊断和治疗数据的比较,一致的阳性和不一致的结果.
分析PCR循环值,中枢液白细胞计数,MRI异常和出院诊断.

主要成果:

在1387名患者中,25人至少在一次测定中呈阳性;11人 (44%) 有不一致的结果.
九名患者是向PCR阳性/MEP阴性,两名是向PCR阴性/MEP阳性.
不一致的向PCR阳性/MEP阴性患者显示了较高的PCR周期值,较低的CSF白细胞计数,较少的MRI异常和较轻的临床表现.

结论:

具有不一致的向PCR阳性/MEP阴性结果的患者表现出较轻微的临床疾病.
这些发现有助于临床医生和实验室人员确定是否需要同时进行针对性和多重的HSV PCR测试.
有助于识别多重PCR单独可能足够的患者,尽管其敏感性较低.

关键词:

中枢神经系统中枢神经系统诊断诊断诊断诊断诊断在HSV的PCR中.简单的疹病毒简单的病毒.多重性脑膜炎/脑炎面板

更多相关视频

Plaquing of Herpes Simplex Viruses

Plaquing of Herpes Simplex Viruses

Published on: November 5, 2021

Author Spotlight: Advancing Immune Monitoring in Critical Care Patients Using Whole Blood Assays

Author Spotlight: Advancing Immune Monitoring in Critical Care Patients Using Whole Blood Assays

Published on: September 20, 2024

相关概念视频

Herpes

Herpes

Herpes simplex type 1 (HSV‑1) is a widespread pathogen responsible for orolabial lesions. It is an enveloped, double-stranded DNA (dsDNA) virus belonging to the family Herpesviridae. Once the virus infects a host cell, its double‑stranded DNA genome is delivered into the nucleus, where a coordinated cascade of immediate‑early, early, and late gene expression directs viral DNA replication, structural protein synthesis, and virion assembly. After primary infection of epithelial cells, HSV-1...

Genital Herpes

Genital Herpes

Genital herpes is a sexually transmitted infection primarily caused by herpes simplex virus type 2 (HSV-2), though herpes simplex virus type 1 (HSV-1) is increasingly implicated in genital infections, particularly among younger populations. Transmission occurs mainly through sexual contact, with asymptomatic viral shedding serving as a major route of spread. This characteristic makes HSV-2 difficult to control at a population level, as individuals may unknowingly transmit the virus even in the...