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在Tilia henryanaana中释放种子休眠状态的方法和生理研究

ChenYin Peng¹, Yu Wu¹, Hao Cai²

¹College of Forestry, Nanjing Forestry University, 159 Longpan Road, Xuanwu District, Nanjing, Jiangsu, 210037, PR China; Co-innovation Center for Sustainable Forestry in Southern China, Southern Tree Inspection Center National Forestry Administration, 159 Longpan Road, Xuanwu District, Nanjing, Jiangsu, 210037, PR China.

Journal of plant physiology

|June 30, 2023

概括

打破罕见的Tilia henryana树种的严重休眠状态对于其繁殖至关重要. 硫酸处理,吉伯酸 (GA3) 和冷分层的组合有效地释放休眠状态,达到98%的发芽率.

科学领域:

植物学和植物科学植物学和植物科学
种子生理学种子生理学
园艺园艺园艺园艺

背景情况:

提利亚亨利亚纳,一个罕见的中国特有树,表现出严重的种子休眠,妨碍其繁殖和保护.
这种休眠的特点是物理 (种子外套) 和生理 (抑制剂) 的障碍,通常称为综合休眠 (PY + PD).

研究的目的:

为了确定Tilia henryana种子的最佳休眠释放程序.
研究与休眠释放和发芽相关的生理和生化变化.

主要方法:

一个L9 (3^4) 直角实验设计被用来优化休眠释放治疗.
处理方法包括硫酸化,吉伯酸 (GA3) 应用,冷分层和发芽温度控制.
生物化学分析的重点是酶活性,激素水平和氨基酸概况.

主要成果:

最佳的休眠释放协议涉及15分钟的H2SO4处理,1gL-1GA3,45天的5°C分层和20°C的发芽,产生98%的发芽率.
休眠释放显著改变了种子的新陈代谢,包括脂肪水解,增加了酸酸酶和粉酶活性,以及增加了酸途径酶活性.
观察到荷尔蒙变化 (增加GA,ZR;减少ABA,IAA) 和氨基酸组成的变化,与生理激活相关.

结论:

物理化 (H2SO4) 和生理处理 (GA3,冷分层) 的结合有效地克服了Tilia henryana种子复杂的休眠状态.
提高种子外的透性和随后的代谢转变,包括从脂肪中调动能量和激素调节,对于成功发芽至关重要.
了解这些休眠破坏机制对于这种罕见的树种的繁殖和保护至关重要.

关键词:

休眠释放放出休眠状态的时间 H ((2) SO ((4) +GA ((3) +冷分层的分层方式正角测试试验在正角测试试验中生理变化生理变化.种子的种子种子.蒂利亚·亨利安娜

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