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评估由GC-MS诱导的肺炎球菌多糖的结构修饰诱导激活,用于联合疫苗

Niraj Shende¹, Abhijeet Karale¹, Prashant Bore¹

¹Research and Development Department, Serum Institute of India Pvt. Ltd, Hadapsar, Pune, Maharashtra, 411028, India.

Carbohydrate research

|June 30, 2023

概括

评估多糖激活对于结合疫苗至关重要. 这项研究优化了肺炎球菌多糖的化,揭示了影响疫苗质量和制造一致性的关键糖激活模式.

科学领域:

疫苗开发疫苗开发
生物化学生物化学
分析化学分析化学

背景情况:

多糖激活是联合疫苗制造的关键质量属性.
5,6B,14,19A和23F血清型的肺炎球菌多糖化物 (PnPs) 是疫苗中的关键成分.

研究的目的:

为了评估在化后特定的肺炎球菌多糖体血清型的激活.
为了将多糖激活与结合动力学和结合性质相关联.
为了确定为一致的疫苗制造提供最佳的化条件.

主要方法:

肺炎球菌多糖化合物 (PnPs) 的化3分钟和8分钟.
聚糖的甲醇化和衍生物化用于气色谱-质谱 (GC-MS) 分析.
使用尺寸排除色谱-高性能液体色谱 (SEC-HPLC) 和使用SEC-多角度光散射 (SEC-MALS) 的绝对摩尔质量的结合动力学的估计.

主要成果:

控制的结合动力学和最佳的摩尔质量被观察到与CRM197载体蛋白.
葡萄糖 (Glc) 和银河糖 (Gal) 是所有PNP血清型中最常被激活的糖类.
特定血清型中的N-乙糖 (PneuNAc,GalNAc,Rha) 在8分钟内显示出>50%的激活,导致结合聚合物的形成.

结论:

对结构变化的GC-MS分析提供了激活多糖的基本特征.
了解糖的激活模式对于控制合聚合物形成至关重要.
优化的化条件确保了肺炎球菌合疫苗制造的一致的质量.

关键词:

激活活动激活活动结合疫苗的结合疫苗化的方法在GC-MS中使用GC-MS.肺炎球菌的多糖化物美国证券交易委员会-HPLC.美国证券交易所 (SEC-MALS)

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相关概念视频

Conjugated Proteins

Conjugated Proteins

Simple proteins and protein complexes contain only amino acids. In contrast, many other proteins, called conjugated proteins, covalently bond with non-protein moieties.
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...