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多种Mycobacterium瘤O-乙转移酶影响糖脂结构和殖民地形态型

Morgane Illouz¹, Louis-David Leclercq², Clara Dessenne²

¹Centre National de la Recherche Scientifique UMR 9004, Institut de Recherche en Infectiologie de Montpellier (IRIM), Université de Montpellier, Montpellier, France.

The Journal of biological chemistry

|June 30, 2023

概括

菌根菌O-乙化,对毒性至关重要,涉及冗余的O-乙转移酶. 乙化损失会改变葡萄糖脂结构并影响感染.

科学领域:

微生物学微生物学
葡萄糖生物学葡萄糖生物学
传染性疾病传染性疾病

背景情况:

菌根杆菌疹会导致严重的肺部感染,其光滑 (S) 和粗 (R) 变体在细胞壁糖脂 (GPL) 中不同.
在S变体中丰富的6-deoxy-α-L-talose (6-dTal) 糖对毒性至关重要,其缺乏导致R形态.
6-dTal O-乙化在M.瘤毒性和GLP结构中的作用尚不清楚.

研究的目的:

为了研究M. abscessus O-乙转移酶 (atf1,atf2,MAB_1725c,MAB_3448) 在GPL.内6-dTal的乙化中的功能.
确定改变的GLP乙化对M. abscessus殖民地形态,毒性和宿主-病原体相互作用的影响.

主要方法:

在M. abscessus.中,为假定的O-乙转移酶 (atf1,atf2) 和它们的类似物 (MAB_1725c,MAB_3448) 创建了基因删除突变物.
使用质谱分析分析了GLP乙化概况.
殖民地形态学和巨细胞内部化试验是在突变菌株上进行的.

主要成果:

删除单个ATF基因并没有显著影响GPL乙化,这表明功能冗余.
三重 (atf1-atf2-MAB_1725c) 和四重 (atf1-atf2-MAB_1725c-MAB_3448) 突变分别显示了逐渐减少和缺失的GL乙化.
突变者积累了超甲基化GPL,并在殖民地形态上表现出微妙的变化,但巨细胞内部化没有受到影响.

结论:

多个功能冗余的O-乙转移酶负责M. abscessus GPL中的6-dTal乙化.
GLP O-乙化影响糖甘生物合成,可能通过调节生物合成流量.
虽然对毒性至关重要,但O-乙化在M. abscessus巨相互作用中的直接作用需要进一步调查.

关键词:

这种细菌是Mycobacterium abscessus.乙烯基转移酶的使用方法细胞壁上的细胞壁.葡萄糖类脂质 (glycopeptidolipid) 是一种巨细胞是一个巨细胞.质谱测量质谱测量质谱测量质谱测量质量测量质谱测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量

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