在细胞分裂过程中,细胞的内部化需要默特克介导的流入
Susumin Yang1,2, Chanhyuk Min1,2, Hyunji Moon1,2
1School of Life Sciences, Gwangju Institute of Science and Technology, Gwangju, 61005, Korea.
Cell death & disease
|June 30, 2023
概括
细胞内的升高对于细胞分裂 (efferocytosis) 至关重要,这是清除亡细胞的过程. 梅特克介导的流入驱动了myosin II收缩和actin分解,确保了有效的细胞尸体内化.
科学领域:
- 细胞生物学 细胞生物学
- 免疫学 免疫学 免疫学
- 信号传递 信号传递
背景情况:
- 细胞分裂,即吞细胞的吞,需要精确的调节,无论是细胞内还是细胞外.
- 虽然细胞内水平在细胞细胞分裂过程中上升,但其在内部化过程中的具体作用仍然基本上未知.
- 默特克受体在细胞分裂中发挥作用,但其对动态和细胞尸体吞的确切贡献尚不清楚.
研究的目的:
- 为了研究细胞内的升高在内部化步骤的作用,efferocytosis.
- 阐明梅特克介导的流影响着细胞杯动态的分子机制.
- 为了确定卡尔莫杜林-MLCK-MLC通路对依赖性乙细胞分裂的贡献.
主要方法:
- 通过遗传和药理方法操纵细胞内水平.
- 使用活细胞成像分析细胞杯的形成,延伸和关闭.
- 评估F-actin动力学,髓轻链酸化和蛋白质与蛋白质相互作用 (Calmodulin-MLCK).
- 使用Mertk缺陷模型和Mertk介导的流入抑制剂.
主要成果:
- 细胞内的显著耗尽,废止了细胞内的细胞内化,延迟了细胞杯的闭合.
- 障碍的F-actin分解和减少卡尔莫杜林与MLCK的相互作用导致MLC酸化的减少,阻碍杯子的关闭.
- 卡尔莫杜林-MLCK-MLC轴或Mertk介导的流的干扰导致因内部化缺陷而导致低效的血细胞分裂.
结论:
- 梅特克介导的细胞内的升高对于在细胞分裂过程中亡细胞的内部化至关重要.
- 细胞内的升高促进了由肌二介导的收缩和F-actin分解,这对于食细胞杯的关闭至关重要.
- 卡尔莫杜林-MLCK-MLC信号通路是在细胞分裂中的作用的关键下游媒介.
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