扩展剂中含有 прополис的纳米脂质体强化对水牛精液冷保存的影响
Sameh A Abdelnour1, Mahmoud A E Hassan2, Ahmed M Shehabeldin2
1Department of Animal Production, Faculty of Agriculture, Zagazig University, Zagazig, 44511, Egypt. saelnour@zu.edu.eg.
Scientific reports
|June 30, 2023
概括
补充水牛精液扩展剂与填充的纳米脂质体 (PRNL) 显著改善了冷保存的精子质量. 最佳度为2μg/mL的PRNL增强了运动性,活力和抗氧化状态,同时减少了氧化应激和亡.
科学领域:
- 动物科学动物科学
- 生殖生物学 生殖生物学
- 生物技术是生物技术.
背景情况:
- 由于对冷伤害的敏感性,水牛精子冷保存面临着挑战.
- 改善低温抵抗对于在水牛中推进辅助生殖技术至关重要.
- 是一种天然树脂,具有抗氧化特性,可以在冷保存期间保护精子.
研究的目的:
- 评估填充纳米脂质体 (PRNL) 对冷保存的水牛精子精液质量的影响.
- 评估PRNL对冷保存的水牛精液中抗氧化状态和亡基因表达的影响.
- 确定PRNL的最佳度,以增强水牛精子的冷抵抗力.
主要方法:
- 准备了PRNL并根据物理化学特性 (大小,泽塔潜力) 进行了表征.
- 牛精液被冷保存在Tris扩展剂中,并补充了不同的PRNL度 (0,2,4,6μg/mL).
- 分析了解后的精液质量参数,包括运动性,活力,膜完整性,染色体损伤,氧化还原状态,亡标志物和基因表达 (Bax,Bcl2,Caspase 3).
主要成果:
- 补充2或4μg/mLPRNL显著改善了精子的渐进性运动性,活力和膜完整性.
- 2μg/mL的PRNL组显示出最少的精子异常和染色质损伤.
- 补充PRNL,特别是2μg/mL,增强了抗氧化活性 (TAC,SOD,GPx,CAT),降低了氧化应激标志物 (ROS,MDA) 和降低了酶3活性.
- 在2μg/mL的PRNL显著降低了Bax和Caspase3基因表达,同时增加了Bcl2表达.
结论:
- 补充水牛精液扩展剂的2或4μg/mLPRNL有效地提高了解后的精子质量.
- 通过提高抗氧化能力,减轻氧化应激,减少水牛精子中的亡,PRNL改善了冷保存结果.
- 建议PRNL的最佳度为2μg/mL,以保持超结构完整性并改善水牛精子的冷抵抗力.
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