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微质和星体细胞中的细胞外囊泡的细胞内贩运差异

Marina Pantazopoulou¹, Agaristi Lamprokostopoulou², Dimitra Sotiria Karampela²

¹Biomedical Research Foundation Academy of Athens-BRFAA, Clinical-Experimental Surgery & Translational Research, 4, Soranou Tou Efesiou Street, 11527, Athens, Greece. mpantazopoulou@bioacademy.gr.

Cellular and molecular life sciences : CMLS

|June 30, 2023

概括

细胞外囊泡 (EVs) 通过细胞或巨细胞细胞形成进入大脑的质细胞,然后在内溶酶体中进行处理. 大脑衍生的EV也将病理性α-synuclein转移到微质,帮助神经退行性疾病中的有毒蛋白质清除.

科学领域:

神经科学是一个神经科学.
细胞生物学细胞生物学
生物化学生物化学

背景情况:

细胞外囊泡 (EVs) 对于中枢神经系统 (CNS) 中的细胞通信至关重要.
大脑细胞中EV吸收和贩运的细胞内途径在很大程度上是未知的.
了解EV处理对于神经退行性疾病研究至关重要.

研究的目的:

为了研究内细胞通路和初级质细胞中EVs的亚细胞分类.
检查EVs在α-synuclein (α-syn) 传播中的作用.
阐明微质细胞和天体细胞中电子电流吸收和贩运的机制.

主要方法:

主要小鼠微质细胞和星球细胞用DI染色小鼠大脑衍生的EVs进行了化.
使用主要内细胞通路的药理抑制剂 (Cytochalasin D,EIPA,胆固醇剥离剂).
通过使用内细胞标志物 (Rab5,Lamp1) 的同位化分析了EV内部化和贩运.
特别评估了与EV相关的纤维状α-syn吸收.

主要成果:

大脑衍生的EV被微质细胞和天体细胞内部化,在微质细胞的效率更高.
电子电路被分类为内溶解体,通过Rab5和Lamp1.1的同位体表示.
抑制细胞/巨细胞减少了EV吸收;胆固醇枯竭改变了内分类.
与EV相关的纤维状α-syn被微质内化并被运输到内分泌体通路.

结论:

EVs主要通过细胞和/或巨细胞被质细胞内部化.
电脑细胞通过质细胞中的内溶酶体路径进行处理.
大脑衍生的EVs调解病理性α-syn向微质细胞的转移,这表明它在神经退行性疾病中清除有毒聚合物的作用.

关键词:

阿尔法-同核素是什么阿尔法-同核素星球细胞是星球细胞.细胞内存症是指细胞内存症.这是内分泌体路径.lysosome 是一个溶解体.微质细胞中的微质细胞小细胞外囊泡 (小细胞外囊泡) (sEVs)

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