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在Picea abies中对多镇压复合体2 (PRC2) 子单元的分析,重点是胚胎发育

Tianqing Zhu¹, Jiwen Hu², Xiaowei Yang²

¹State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Haidian District, Dongxiaofu 1, Beijing, 100091, People's Republic of China.

BMC plant biology

|June 30, 2023

概括

这项研究确定了挪威杉 (Picea abies) 中的多抑制复合体2 (PRC2) 基因,揭示了它们在种子和胚胎发育中的作用. 这项研究促进了对针叶树胚胎发生和细胞重编程的理解.

科学领域:

植物分子生物学植物分子生物学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
发育生物学是发展生物学.

背景情况:

聚合体抑制复合体2 (PRC2) 对动物和植物的转录抑制和细胞分化至关重要.
在更高的植物中,PRC2子单元的演化方式各不相同,但对于体类植物,信息是缺乏的.

研究的目的:

为了表征PRC2核心组分基因在体育种子Picea abies.
研究这些基因在发育过程中的进化关系和表达模式.

主要方法:

在Picea abies中鉴定和克隆PRC2组件的基因.
遗传学和蛋白质领域分析.
在各种胚胎阶段进行量化转录水平测量.
针对H3K27me3定位的免疫组织化学.

主要成果:

在P. abies. 中发现了PRC2成分的同类物 (PaFIE,PaMSI1a/b,PaKMT6A2/4,PaEMF2).
PaMSI1b和PaKMT6A4与胚胎发生有关; PaKMT6A2和PaEMF2在胚胎到种子的过渡中.
在种子发育过程中,H3K27me3在水系区域被丰富.

结论:

这是PRC2核心基因在针叶植物物种中的首次表征.
这些发现提供了关于针叶树在种子/胚胎发育过程中细胞重编程的见解.
允许未来研究针叶树的胚胎潜力和发展.

关键词:

H3K27me3 在线阅读挪威杉是一种挪威杉.聚合组组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组合组种子的种子开发.实体胚胎发生体质胚胎发生.

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