菌体基因组工程用于菌体治疗,以对抗细菌抗菌素耐药性,作为抗生素的替代品
Sani Sharif Usman1,2, Abdullahi Ibrahim Uba3, Evangeline Christina4
1Department of Molecular Biology and Genetic Engineering, School of Bioengineering and Biosciences, Lovely Professional University, Jalandhar-Delhi G.T. Road, Phagwara, 144401, Punjab, India.
Molecular biology reports
|July 1, 2023
概括
使用CRISPR/Cas9等工具的菌体工程为抗生素提供了一个有希望的替代方案,用于对抗多药耐药细菌. 这种方法增强了菌体疗法.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 生物技术是生物技术.
背景情况:
- 过度使用抗生素导致多药耐药 (MDR) 细菌的增加,导致全球数百万人死亡,并对公共卫生构成重大威胁.
- 细菌菌体 (菌体),病毒感染细菌,正在探索作为一种强大的替代传统抗生素.
- 菌体疗法由于其有效性和安全性而具有前景,但需要解决诸如狭窄的宿主范围和耐药性等局限性.
研究的目的:
- 审查菌体基因组工程技术的当前趋势和进展.
- 突出菌体疗法的潜力,作为抗生素的可行替代品,用于打击抗菌素耐药性 (AMR).
- 讨论菌体工程如何克服当前菌体治疗的局限性.
主要方法:
- 对菌体基因组工程技术的当前文献的综述.
- 探索分子生物学工具,如合成生物学,同源重组和向核酶 (CRISPR/Cas9,ZFNs,TALENs) 等.
- 对菌体治疗模型和人类研究的分析.
主要成果:
- 菌体工程,特别是使用CRISPR/Cas9,可以操纵菌体基因组以增强抗菌活性.
- 工程菌体有可能克服诸如狭窄的宿主范围和细菌耐药性等局限性.
- 基于菌体的疗法在各种临床前和临床环境中显示出有希望的疗效和安全性.
结论:
- 菌体基因组工程对于优化菌体治疗来对抗AMR至关重要.
- 克里斯普尔/Cas9和其他先进技术是开发有效的菌体治疗的关键.
- 通过基因工程增强的菌体疗法代表了抗生素对抗MDR病原体的强大替代品.
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