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在工程化Yarrowia lipolytica中促进Geranyl二酸盐合成以生产Linalool

Maria O Taratynova¹, Ekaterina E Tikhonova², Iuliia M Fedyaeva²

¹NRC "Kurchatov Institute", Kurchatov Genomic Center, sq. Academician Kurchatova, 1, Moscow, 123182, Russia. taratynovamary@gmail.com.

Applied biochemistry and biotechnology

|July 1, 2023

概括

工程酵母Yarrowia lipolytica现在可以产生林纳醇. 这项研究通过优化代谢途径和使用CRISPR-Cas9技术用于工业应用来增强生产.

科学领域:

生物技术是生物技术.
代谢工程是代谢工程.
合成生物学合成生物学

背景情况:

在花精油中发现的一种单类物质linalool,具有宝贵的生物特性.
其重要的商业潜力在于食品和香水行业.
在微生物中新生产的林纳醇对于可持续的采购是可取的.

研究的目的:

将油性酵母Yarrowia lipolytica改造为新的林纳醇生产.
通过操纵前体路径和酶表达来提高林纳醇产量.
研究特定遗传修饰对林纳醇积累的影响.

主要方法:

来自Actinidia argute的 (S) - 林醇合成酶 (LIS) 基因过度表达.
通过使用突变ERG20^F88W-N119W和Catharanthus roseus CrGPPS的工程代谢流向吉拉尼尔二酸盐 (GPP).
通过CRISPR-Cas9.9破坏本源的二甲基糖醇激酶 (DGK1) 基因.

主要成果:

在Yarrowia lipolytica.中实现了新的林纳醇生产.
工程菌株使用糖作为碳来源积累了109.6毫克/升的林纳醇.
与ERG20^F88W-N119W相比,CrGPPS表达在增加林纳醇积累方面表现出更高的效率.

结论:

雅罗维亚脂质菌可以成功地被设计为新的林纳醇生物合成.
代谢工程策略,特别是增强GPP供应,对于促进林纳醇生产至关重要.
这项工作为微生物生产工业应用的林纳醇提供了基础.

关键词:

杰拉尼尔二酸盐合成酶的使用林娜洛尔 (Linalool) 是一个名为林娜洛尔 (Linalool) 的名字.代谢工程是代谢工程.单聚类是单聚类的.雅罗维亚 (Yarrowia lipolytica) 是一个高脂的植物.

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