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Updated: Jul 24, 2025

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热冲击转录因子家族的全基因组识别,分类和表达分析转换草 (Panicum virgatum L.) 的热冲击转录因子家族

Kunliang Xie¹, Jinliang Guo¹, Shaoyu Wang¹

¹College of Agronomy, Northwest A&F University, Yangling, Shaanxi 712100, China; State Key Laboratory of Crop Stress Biology for Arid Areas, Yangling, Shaanxi, 712100, China.

Plant physiology and biochemistry : PPB

|July 1, 2023

概括

这项研究在草中确定了48个热冲击转录因子 (Hsfs),揭示了PvHsf03和PvHsf25等关键基因与热应激反应和耐受性有关.

科学领域:

植物科学植物科学
分子生物学分子生物学
生物能源作物生物能源作物

背景情况:

切换草是一种重要的生物能源作物,在恶劣的环境中壮成长.
热冲击转录因子 (Hsfs) 对于植物应激适应至关重要.
在交叉草中Hsfs的具体作用仍然在很大程度上是未知的.

研究的目的:

为了识别切换草 (Panicum virgatum) 中的HSF基因家族.
阐明PvHsfs在热应力信号和耐受性中的功能性作用.
为增强交换草的应力耐受性提供基础.

主要方法:

用于HSF基因识别和特征的生物信息分析.
遗传学分析将PvHsfs分为A,B和C类.
在热应激下使用RT-PCR分析PvHsf基因表达.
在Arabidopsis中对PvHsf03的子宫外表达,以评估耐热性.

主要成果:

有48个PvHsf基因被确定,分为HSfA,HSfB和HSfC等级.
PvHsfs具有N端DNA结合域和多样化的染色体分布.
促成体分析揭示了压力和激素反应的cis元素;细分重复驱动家庭扩张.
PvHsf03和PvHsf25分别对早期和晚期热应激反应至关重要.
一般来说,hsfB家族显示出热应激反应的负调节.
宫外表达PvHsf03增强了Arabidopsis的耐热性.

结论:

这项研究建立了交换草HSFS的全面目录.
PvHsf03和PvHsf25是交换草中热应激反应的关键调节者.
这些发现促进了生物能源作物的耐热性遗传改进.

关键词:

阿拉比多普西斯 (Arabidopsis) 是一种植物.全基因组范围的基因组.热冲击转录因子的转录因子热应激是一种热应激.切换草地切换草地

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