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由Bacillus cereus L17合成的poly-β-hydroxybutyrate的响应表面优化,使用乙酸作为碳源

Zhaosong Huang¹, Boya Liang¹, Fang Wang¹

¹School of Biological Science and Technology, University of Jinan, Jinan, China.

International journal of biological macromolecules

|July 1, 2023

概括

研究人员从废水中选了Bacillus cereus L17,以产生聚酸酸盐 (PHA). 优化的发酵条件显著增加了35.2%的PHA产量,突出了其对工业应用的潜力.

科学领域:

微生物学微生物学
生物技术是生物技术.
聚合物科学聚合物科学

背景情况:

废水处理从活性污泥中产生挥发性脂肪酸 (VFA).
聚酸酸盐 (PHAs) 是一种可生物降解的聚合物,具有多种应用.
从工业废物中选能够产生PHA的微生物对于可持续的生物制造至关重要.

研究的目的:

从挥发性脂肪酸中分离和识别一种能够从挥发性脂肪酸中产生多酸酸盐 (PHA) 的Bacillus菌株.
描述合成的PHA,并优化发酵条件以提高产量.
评估已识别的菌株及其产品的工业和医疗用途潜力.

主要方法:

从氧化肥废水的活性污泥中选细菌菌株L17.
使用16S rRNA测序和植物遗传学分析识别菌株.
使用各种方法对生产的聚β-基酸盐 (PHB) 进行表征.
通过单因素实验,普莱克特-伯曼,盒子-贝恩肯设计和响应表面方法来优化发酵条件.

主要成果:

鉴定出Bacillus cereus L17是一种能够耐受10g/L酸的菌株,并且能够产生PHA.
合成的聚合物被描述为具有理想性质的聚β-基酸盐 (PHB):晶度低,可塑性好,热稳定性高,聚分散性低.
优化的发酵条件 (初始pH6.7,温度25°C,装载量124毫升) 导致PHB产量增加35.2%.
生产的PHB展示了热塑性应用的广泛操作范围.

结论:

细菌花L17是一种有前途的微生物,可以从工业废水副产品中生产高质量的聚β-基酸盐.
优化发酵显著提高PHB产量,使该过程更具经济可行性.
具有特征的PHB具有适用于各种工业和医疗应用的特性,有助于可持续的生物聚合物生产.

关键词:

聚-β-基丁酸盐-基酸盐响应表面优化响应表面优化一个因素优化优化.

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