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工程大肠杆菌 (Escherichia coli) 从乙烯糖醇中生产芳香化学物质

Smaranika Panda¹, Jie Fu J Zhou¹, Michelle Feigis²

¹Department of Chemical and Biomolecular Engineering, National University of Singapore, Singapore.

Metabolic engineering

|July 1, 2023

概括

这项研究工程师埃斯切里希亚大肠杆菌转化乙烯糖醇 (EG),来自塑料废弃物,到有价值的芳香化学物质,如L-tyrosine. 这种微生物生产方法显示出从非食品原料中实现可持续化学合成的前景.

科学领域:

生物技术是生物技术.
代谢工程是代谢工程.
合成生物学合成生物学

背景情况:

微生物生产芳香化学品在工业上非常重要.
目前的方法主要使用糖或甘油作为碳来源.
从废物中获得的乙烯糖醇 (EG) 是一种替代原料.

研究的目的:

为了设计大肠杆菌的微生物转化乙烯糖醇 (EG) 成芳香化学物质.
为了证明使用EG作为L-tyrosine,L-phenylalanine和p-coumaric酸生产的初级碳基质的可行性.
为了验证从回收聚乙烯二甲酸 (PET) 废物中获得的EG的转化.

主要方法:

大肠杆菌的新陈代谢工程.
使用乙烯糖醇作为唯一的碳来源进行发酵.
废弃PET的酸性水解以获得EG.

主要成果:

改造后的大肠杆菌从10g/L EG中产生了2g/L的L-tyrosine,表现优于葡萄糖.
从EG成功合成了L-phenylalanine和p-coumaric酸. 从EG成功合成了L-phenylalanine和p-coumaric酸.
从废弃物衍生的EG和商业EG中获得了可比的L-氨酸位.

结论:

大肠杆菌可以通过代谢工程来有效地从乙烯基醇中产生芳香化学物质.
这种方法为从塑料和纤维素废物中生产有价值的化合物提供了一个可持续的途径.
开发的微生物菌株为社区在芳香化学合成中提供了宝贵的工具.

关键词:

芳香化学品是一种芳香化学品.埃舍里希亚大肠杆菌 (Escherichia coli) 是一个大肠杆菌.乙烯糖醇是一种乙烯基醇.在L-tyrosine的使用中.代谢工程是代谢工程.聚乙烯二甲酸盐 (PET) 是一种聚乙烯二甲酸盐.

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