生物技术产生的氨酸下游工艺强化:净化和表征
Priya Shukla1, Pradeep Srivastava1, Abha Mishra1
1School of Biochemical Engineering, Indian Institute of Technology (Banaras Hindu University), 221005 Varanasi, India.
Journal of bioscience and bioengineering
|July 1, 2023
概括
这项研究提出了一种有效的方法,用于从发酵中净化氨酸 (HA). 优化的工艺产生了高纯度的HA,适合用于制药应用.
科学领域:
- 生物化学 生物化学
- 生物技术是生物技术.
- 材料科学 材料科学 材料科学
背景情况:
- 氨酸 (HA) 是一种葡萄糖氨基酸,具有多种不同的临床应用.
- 有效的净化方法对于最大限度地提高HA的回收和纯度至关重要.
- 菌株Streptococcus zooepidemicus发酵是微生物HA生产的常见来源.
研究的目的:
- 为了研究和优化下游净化酸的净化技术.
- 为了实现从发酵中最大限度地回收和高纯度的HA.
- 为了验证净化HA的纯度和结构完整性.
主要方法:
- 化Streptococcus zooepidemicus MTCC 3523的发酵,以产生HA.
- 多步净化包括过,吸附 (活性炭,XAD-7树脂) 和透过.
- 使用富里埃变换红外光谱学,X射线衍射,核磁共振和扫描电子显微镜进行表征.
- 抗氧化活性测定包括DPPH激素清除,总抗氧化能力,基激素清除和降低功率.
主要成果:
- 用活性炭和XAD-7树脂去除了核酸和高分子量蛋白质.
- 不溶性和低分子量杂质被有效地通过过清除.
- 实现了79.16%的HA回收,纯度接近90%.
- 通过各种分析技术验证了HA的存在,纯度和结构.
- 已证明具有显著的抗氧化作用:DPPH激素清除 (4.87 ± 0.45 kmol TE/g),总抗氧化能力 (13.32 ± 0.52%),基激素清除 (32.03 ± 0.12%) 和降解功率 (24.85 ± 0.45%).
结论:
- 沉,吸附和透是从发酵中提取HA的有效方法.
- 优化的工艺产生了适合非注射应用的药品级氨酸.
- 纯化的HA具有显著的抗氧化特性.
相关概念视频
01:27Upstream Processing
Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...
01:29Downstream Processing
Downstream processing begins once fermentation is complete and involves a series of steps to recover and purify products such as acids, vitamins, antibiotics, or proteins.Cell HarvestingFor example, for intracellular protein-based products, the first step is harvesting the cells. This is typically achieved using centrifugation or filtration to separate the cells from the liquid phase.Cell Disruption for Intracellular ProductsIf the target product is intracellular, the harvested cells must be...